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- W1489754222 abstract "The trans-Golgi network is one of the main sorting stations for proteins and lipids in the eukaryotic endomembrane system. Yet, the mechanisms for sorting and transport out of the trans-Golgi network remain poorly understood. In this work, an in vitro procedure to reconstitute carrier formation at the trans-Golgi was established. Using this assay, it could be shown that formation of carriers containing the asialoglycoprotein receptor H1 and the cation dependent mannose 6-phosphate receptor is independent of the clathrin machinery, but dependent on GTP hydrolysis. This is in contrast to the common notion that the mannose 6phosphate receptor exits the Golgi in clathrin coated vesicles, but is consistent with recent evidence for tubular export carriers or maturation. The assay is based on sulfation, which can only take place in the trans-Golgi and thus defines the starting compartment. To improve the radioactive sulfation signal in the assay, a novel tag was developed that contains an attachment site for glycosaminoglycans (long, linear and heavily sulfated carbohydrate polymers). To investigate the influence of glycosaminoglycan attachment on protein sorting, biosynthetic exocytosis, endocytosis, endocytic recycling, and overall stability of H1 with or without glycan was examined. While stability and recycling were unaffected, exocytosis was accelerated and endocytosis was slowed down by carbohydrate attachment. Endocytosis was impaired due to interactions of glycosaminoglycan with the extracellular matrix, but the alteration of exocytosis rate requires further investigation, as it suggests that the glycan diverts the protein to a different sorting route." @default.
- W1489754222 created "2016-06-24" @default.
- W1489754222 creator A5043857543 @default.
- W1489754222 date "2007-01-01" @default.
- W1489754222 modified "2023-09-23" @default.
- W1489754222 title "In vitro reconstitution of trans-Golgi exit and the effect of GAG attachment on protein sorting" @default.
- W1489754222 doi "https://doi.org/10.5451/unibas-004641752" @default.
- W1489754222 hasPublicationYear "2007" @default.
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