Matches in SemOpenAlex for { <https://semopenalex.org/work/W1489771292> ?p ?o ?g. }
- W1489771292 endingPage "242" @default.
- W1489771292 startingPage "235" @default.
- W1489771292 abstract "Toxic cyanobacterial blooms in freshwater have been considered as threats to human health. Microcystins are a family of cyclic polypeptides produced by cyanobacteria and are toxic to plants and animals. Microcystin-LR (MC-LR) is the most toxic variant among the microcystin family and could cause oxidative stress in various organs, including the reproduction system. The aim of this study was to investigate the effect of MC-LR on apoptosis of Sertoli cells that play an essential role in the development and maturation of sperm cells. Sertoli cells were isolated from healthy immature rats and cultured with MC-LR. The viability of Sertoli cells was decreased after treatment with MC-LR at 10 μg/ml for 24 h (P < 0.05). Moreover, the MC-LR-treated cells exhibited condensed chromatin and fragmented nuclei, features of apoptosis, as judged by Hoechst 33258 staining. We also analyzed the mRNA and protein levels of three apoptosis-related genes, p53, bax and bcl-2, using reverse transcription-polymerase chain reaction and Western blot analyses, respectively. Both p53 and bax function as promoters of apoptosis, while bcl-2 is an apoptotic suppressor. The mRNA and protein expression levels of p53 and bax were increased in Sertoli cells treated with MC-LR at 10 μg/ml compared with the control group (P < 0.05), while the bcl-2 protein levels were decreased in cells treated with MC-LR at 10 μg/ml (P < 0.05). Moreover, caspase-3 activity that is involved in the induction of apoptosis was significantly increased in Sertoli cells treated with MC-LR. These results indicate that MC-LR induces apoptosis of Sertoli cells." @default.
- W1489771292 created "2016-06-24" @default.
- W1489771292 creator A5005614740 @default.
- W1489771292 creator A5005689660 @default.
- W1489771292 creator A5016845866 @default.
- W1489771292 creator A5031458979 @default.
- W1489771292 creator A5084743187 @default.
- W1489771292 creator A5091387485 @default.
- W1489771292 date "2011-01-01" @default.
- W1489771292 modified "2023-10-17" @default.
- W1489771292 title "A Cyanobacterial Toxin, Microcystin-LR, Induces Apoptosis of Sertoli Cells by Changing the Expression Levels of Apoptosis-Related Proteins" @default.
- W1489771292 cites W1492302205 @default.
- W1489771292 cites W1544032942 @default.
- W1489771292 cites W1598082435 @default.
- W1489771292 cites W1621124914 @default.
- W1489771292 cites W1970046714 @default.
- W1489771292 cites W1972331889 @default.
- W1489771292 cites W1973734439 @default.
- W1489771292 cites W1981883990 @default.
- W1489771292 cites W1984842291 @default.
- W1489771292 cites W1991865867 @default.
- W1489771292 cites W1997511772 @default.
- W1489771292 cites W1999076914 @default.
- W1489771292 cites W2003069819 @default.
- W1489771292 cites W2004256319 @default.
- W1489771292 cites W2008878988 @default.
- W1489771292 cites W2011727075 @default.
- W1489771292 cites W2014004316 @default.
- W1489771292 cites W2022393030 @default.
- W1489771292 cites W2026366854 @default.
- W1489771292 cites W2027575456 @default.
- W1489771292 cites W2030524684 @default.
- W1489771292 cites W2032308096 @default.
- W1489771292 cites W2032903606 @default.
- W1489771292 cites W2033847504 @default.
- W1489771292 cites W2034246810 @default.
- W1489771292 cites W2034395844 @default.
- W1489771292 cites W2045563337 @default.
- W1489771292 cites W2062844242 @default.
- W1489771292 cites W2064248967 @default.
- W1489771292 cites W2065933981 @default.
- W1489771292 cites W2080089609 @default.
- W1489771292 cites W2085143181 @default.
- W1489771292 cites W2088119581 @default.
- W1489771292 cites W2101091211 @default.
- W1489771292 cites W2103965206 @default.
- W1489771292 cites W2105620485 @default.
- W1489771292 cites W2105839948 @default.
- W1489771292 cites W2106713305 @default.
- W1489771292 cites W2107468514 @default.
- W1489771292 cites W2109992783 @default.
- W1489771292 cites W2111650534 @default.
- W1489771292 cites W2114918609 @default.
- W1489771292 cites W2134979684 @default.
- W1489771292 cites W2140327099 @default.
- W1489771292 cites W2140464143 @default.
- W1489771292 cites W2153385508 @default.
- W1489771292 cites W2161269223 @default.
- W1489771292 cites W2325707043 @default.
- W1489771292 cites W4239718055 @default.
- W1489771292 doi "https://doi.org/10.1620/tjem.224.235" @default.
- W1489771292 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21701130" @default.
- W1489771292 hasPublicationYear "2011" @default.
- W1489771292 type Work @default.
- W1489771292 sameAs 1489771292 @default.
- W1489771292 citedByCount "26" @default.
- W1489771292 countsByYear W14897712922012 @default.
- W1489771292 countsByYear W14897712922013 @default.
- W1489771292 countsByYear W14897712922014 @default.
- W1489771292 countsByYear W14897712922015 @default.
- W1489771292 countsByYear W14897712922016 @default.
- W1489771292 countsByYear W14897712922017 @default.
- W1489771292 countsByYear W14897712922018 @default.
- W1489771292 countsByYear W14897712922019 @default.
- W1489771292 countsByYear W14897712922020 @default.
- W1489771292 countsByYear W14897712922022 @default.
- W1489771292 countsByYear W14897712922023 @default.
- W1489771292 crossrefType "journal-article" @default.
- W1489771292 hasAuthorship W1489771292A5005614740 @default.
- W1489771292 hasAuthorship W1489771292A5005689660 @default.
- W1489771292 hasAuthorship W1489771292A5016845866 @default.
- W1489771292 hasAuthorship W1489771292A5031458979 @default.
- W1489771292 hasAuthorship W1489771292A5084743187 @default.
- W1489771292 hasAuthorship W1489771292A5091387485 @default.
- W1489771292 hasBestOaLocation W14897712921 @default.
- W1489771292 hasConcept C104317684 @default.
- W1489771292 hasConcept C105580179 @default.
- W1489771292 hasConcept C123765429 @default.
- W1489771292 hasConcept C134018914 @default.
- W1489771292 hasConcept C153911025 @default.
- W1489771292 hasConcept C16685009 @default.
- W1489771292 hasConcept C190283241 @default.
- W1489771292 hasConcept C2776415932 @default.
- W1489771292 hasConcept C2781068068 @default.
- W1489771292 hasConcept C55493867 @default.
- W1489771292 hasConcept C71924100 @default.
- W1489771292 hasConcept C86803240 @default.
- W1489771292 hasConcept C95444343 @default.