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• W1489802358 abstract "High-throughput approaches for gene cloning and expression require the development of new, nonstandard tools for use by molecular biologists and biochemists. We have developed and implemented a series of methods that enable the production of expression constructs in 96-well plate format. A screening process is described that facilitates the identification of bacterial clones expressing soluble protein. Application of the solubility screen then provides a plate map that identifies the location of wells containing clones producing soluble proteins. A series of semi-automated methods can then be applied for validation of solubility and production of freezer stocks for the protein production group. This process provides an 80% success rate for the identification of clones producing soluble protein and results in a significant decrease in the level of effort required for the labor-intensive components of validation and preparation of freezer stocks. This process is customized for large-scale structural genomics programs that rely on the production of large amounts of soluble proteins for crystallization trials." @default.
• W1489802358 created "2016-06-24" @default.
• W1489802358 creator A5079815909 @default.
• W1489802358 creator A5080700063 @default.
• W1489802358 creator A5087441712 @default.
• W1489802358 date "2006-04-05" @default.
• W1489802358 modified "2023-10-17" @default.
• W1489802358 title "Strategies for High-Throughput Gene Cloning and Expression" @default.
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• W1489802358 doi "https://doi.org/10.1007/0-387-25856-6_10" @default.
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• W1489802358 hasPublicationYear "2006" @default.
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