FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1489804071> ?p ?o ?g. }

• W1489804071 abstract "Interphotoreceptor retinol-binding protein (IRBP), composed of two or four homologous modules in tandem, plays an important role in retinoid trafficking between the retinal pigmented epithelium, photoreceptors, and Müller cells. The exact nature of this role is not yet clear. Attempts to purify the full-length retinal IRBP to homogeneity for crystallization purposes have largely been unsuccessful, owing primarily to instability and denaturation of the protein at high concentrations in aqueous media.A bacterial expression system was used for the production of the recombinant full-length four modules-containing Xenopus IRBP (xIRBP; 1197 amino acids; 131 kDa). An optimized purification strategy and the presence of molar excesses of a thiol-based reducing agent yielded highly pure xIRBP in a soluble, stable and active form, free of its fusion partner. Binding of all-trans retinol was characterized by fluorescence spectroscopy monitoring ligand-fluorescence enhancement, quenching of endogenous protein fluorescence, and energy transfer.We grew the first diffraction-quality crystal of xIRBP. We have gathered diffraction data from these crystals to 2.46 A resolution, sufficient to yield an atomic model of the tertiary structure of IRBP. Retinol-binding results determined by fluorescence spectroscopy show roughly one retinol-binding site per polypeptide chain.The binding stoichiometry taken together with modeling data suggest that not all modules are functionally equivalent. Determination of the full-length IRBP structure will be a significant breakthrough in understanding the functional roles of IRBP in the visual cycle. The advances presented here will not only lead to the structure of the full-length IRBP, but will allow us to understand how the modules interact in the function of IRBP. Furthermore, these studies will allow characterization of the ligand-binding site(s) with bound ligand(s)." @default.
• W1489804071 created "2016-06-24" @default.
• W1489804071 creator A5027436784 @default.
• W1489804071 creator A5031310707 @default.
• W1489804071 creator A5072685925 @default.
• W1489804071 creator A5073349983 @default.
• W1489804071 date "2007-12-13" @default.
• W1489804071 modified "2023-09-23" @default.
• W1489804071 title "Purification of the full-length Xenopus interphotoreceptor retinoid binding protein and growth of diffraction-quality crystals." @default.
• W1489804071 cites W1486088521 @default.
• W1489804071 cites W1491897518 @default.
• W1489804071 cites W1497157496 @default.
• W1489804071 cites W1508509665 @default.
• W1489804071 cites W1510752969 @default.
• W1489804071 cites W1527872783 @default.
• W1489804071 cites W1546961042 @default.
• W1489804071 cites W1602141111 @default.
• W1489804071 cites W1968459833 @default.
• W1489804071 cites W1976071843 @default.
• W1489804071 cites W1977060467 @default.
• W1489804071 cites W1996442995 @default.
• W1489804071 cites W1996897401 @default.
• W1489804071 cites W2003621204 @default.
• W1489804071 cites W2007208973 @default.
• W1489804071 cites W2007715902 @default.
• W1489804071 cites W2014694459 @default.
• W1489804071 cites W2017254187 @default.
• W1489804071 cites W2018700534 @default.
• W1489804071 cites W2022592544 @default.
• W1489804071 cites W2038264300 @default.
• W1489804071 cites W2046200610 @default.
• W1489804071 cites W2048188877 @default.
• W1489804071 cites W2048208037 @default.
• W1489804071 cites W2054326088 @default.
• W1489804071 cites W2080985035 @default.
• W1489804071 cites W2081694019 @default.
• W1489804071 cites W2088345052 @default.
• W1489804071 cites W2119159503 @default.
• W1489804071 cites W2137588011 @default.
• W1489804071 cites W2146216956 @default.
• W1489804071 cites W2150767772 @default.
• W1489804071 cites W2152448366 @default.
• W1489804071 cites W2165346106 @default.
• W1489804071 cites W2183429871 @default.
• W1489804071 cites W85247325 @default.
• W1489804071 cites W2442689229 @default.
• W1489804071 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2185538" @default.
• W1489804071 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18079675" @default.
• W1489804071 hasPublicationYear "2007" @default.
• W1489804071 type Work @default.
• W1489804071 sameAs 1489804071 @default.
• W1489804071 citedByCount "3" @default.
• W1489804071 countsByYear W14898040712015 @default.
• W1489804071 crossrefType "journal-article" @default.
• W1489804071 hasAuthorship W1489804071A5027436784 @default.
• W1489804071 hasAuthorship W1489804071A5031310707 @default.
• W1489804071 hasAuthorship W1489804071A5072685925 @default.
• W1489804071 hasAuthorship W1489804071A5073349983 @default.
• W1489804071 hasConcept C104317684 @default.
• W1489804071 hasConcept C121332964 @default.
• W1489804071 hasConcept C123894998 @default.
• W1489804071 hasConcept C12554922 @default.
• W1489804071 hasConcept C131667965 @default.
• W1489804071 hasConcept C13965031 @default.
• W1489804071 hasConcept C153064111 @default.
• W1489804071 hasConcept C158711907 @default.
• W1489804071 hasConcept C178790620 @default.
• W1489804071 hasConcept C185592680 @default.
• W1489804071 hasConcept C203036418 @default.
• W1489804071 hasConcept C2776923230 @default.
• W1489804071 hasConcept C2776940978 @default.
• W1489804071 hasConcept C2779535977 @default.
• W1489804071 hasConcept C2779573558 @default.
• W1489804071 hasConcept C2780827179 @default.
• W1489804071 hasConcept C40767141 @default.
• W1489804071 hasConcept C55493867 @default.
• W1489804071 hasConcept C62520636 @default.
• W1489804071 hasConcept C8010536 @default.
• W1489804071 hasConcept C86803240 @default.
• W1489804071 hasConcept C91881484 @default.
• W1489804071 hasConcept C97749175 @default.
• W1489804071 hasConceptScore W1489804071C104317684 @default.
• W1489804071 hasConceptScore W1489804071C121332964 @default.
• W1489804071 hasConceptScore W1489804071C123894998 @default.
• W1489804071 hasConceptScore W1489804071C12554922 @default.
• W1489804071 hasConceptScore W1489804071C131667965 @default.
• W1489804071 hasConceptScore W1489804071C13965031 @default.
• W1489804071 hasConceptScore W1489804071C153064111 @default.
• W1489804071 hasConceptScore W1489804071C158711907 @default.
• W1489804071 hasConceptScore W1489804071C178790620 @default.
• W1489804071 hasConceptScore W1489804071C185592680 @default.
• W1489804071 hasConceptScore W1489804071C203036418 @default.
• W1489804071 hasConceptScore W1489804071C2776923230 @default.
• W1489804071 hasConceptScore W1489804071C2776940978 @default.
• W1489804071 hasConceptScore W1489804071C2779535977 @default.
• W1489804071 hasConceptScore W1489804071C2779573558 @default.
• W1489804071 hasConceptScore W1489804071C2780827179 @default.
• W1489804071 hasConceptScore W1489804071C40767141 @default.
• W1489804071 hasConceptScore W1489804071C55493867 @default.
• W1489804071 hasConceptScore W1489804071C62520636 @default.

• next