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- W1490222790 startingPage "817" @default.
- W1490222790 abstract "cis-Platinum compounds, which are used in cancer chemotherapy, are thought to exert their effect by damaging DNA. It is known that this damage is partially repaired in Escherichia coli. Using cis-Pt-treated pBR322 DNA as a probe, we investigated the role of nucleotide excision repair in the removal of Pt-DNA adducts. We found that the nucleotide excision pathway was the major mechanism for repairing Pt adducts in transforming plasmid DNA but that a recA-dependent pathway also contributed to plasmid survival. When cis-Pt-damaged pBR322 was treated with the purified nucleotide excision enzyme ABC excinuclease in vitro, a fraction of the adducts was removed by the enzyme; this removal resulted in a corresponding increase in transformation efficiency." @default.
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- W1490222790 date "1985-09-01" @default.
- W1490222790 modified "2023-09-23" @default.
- W1490222790 title "Repair of cis-platinum-DNA adducts by ABC excinuclease in vivo and in vitro" @default.
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- W1490222790 doi "https://doi.org/10.1128/jb.163.3.817-823.1985" @default.
- W1490222790 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/219204" @default.
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