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- W1490279667 endingPage "23" @default.
- W1490279667 startingPage "23" @default.
- W1490279667 abstract "For a long time, the human lower airways were considered a sterile environment where the presence of microorganisms, typically revealed by culturing, was interpreted as an abnormal health state. More recently, high-throughput sequencing-based studies have led to a shift in this perception towards the notion that even in healthy conditions the lower airways show either transient presence or even permanent colonization by microorganisms. However, challenges related to low biomass and contamination in samples still remain, and the composition, structure and dynamics of such putative microbial communities are unclear. Here, we review the evidence for the presence of microbial communities in the human lower airways, in healthy subjects and within the context of medical conditions of interest. We also provide an overview of the methodology pertinent to high-throughput sequencing studies, specifically those based on amplicon sequencing, including a discussion of good practices and common pitfalls." @default.
- W1490279667 created "2016-06-24" @default.
- W1490279667 creator A5004201556 @default.
- W1490279667 creator A5008954993 @default.
- W1490279667 creator A5014796391 @default.
- W1490279667 creator A5022142463 @default.
- W1490279667 creator A5040033494 @default.
- W1490279667 creator A5079537064 @default.
- W1490279667 date "2015-01-01" @default.
- W1490279667 modified "2023-10-18" @default.
- W1490279667 title "The microbiome of the human lower airways: a next generation sequencing perspective" @default.
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