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- W1490281572 abstract "The open reading frame encoding the avirulence geneavrPpiA1, previously cloned from race 2 ofPseudomonas syringaepv.pisi, has been subcloned and shown to express peptides corresponding to all six possible start codons. The half-life of the peptides was found to be approximately 5 min inEscherichia coli. Transposon insertions which inactivate the gene inP. syringaepv.pisiwere located both within the open reading frame and in the upstream regulatory sequence, thehrp-box. Homologues of the gene were present only in races 5 and 7, which expressed the avirulence phenotype. The gene was present on the chromosome in all race 2 isolates tested, but was plasmid-borne in the type strains of races 5 and 7. In contrast to the homologueavrRpm1cloned fromP. syringaepv.maculicola, marker-exchange mutagenesis of the gene in races 2 and 7 ofP. syringaepv.pisiwas not found to affect pathogenicity towards the susceptible host as indicated by symptom development or bacterial growth in the plant." @default.
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- W1490281572 date "1997-04-01" @default.
- W1490281572 modified "2023-09-23" @default.
- W1490281572 title "Avirulence geneavrPpiAfromPseudomonas syringaepv.pisiis not required for full virulence on pea" @default.
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- W1490281572 doi "https://doi.org/10.1006/pmpp.1997.0083" @default.
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