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• W1490346887 abstract "Publisher Summary The in vivo axonal transport of intermediate filament (IF) proteins can be done with the measurement of pulse radiolabeling analyses in retinal ganglion cells (RGCs) after the intravitreal injection of [ 35 S]methionine. Defects in axonal transport can cause motor neuron diseases and neuropathies as shown in the case of gene mutations/dysfunctions for the components of the microtubule-based transport in subsets of mouse models and human cases. This chapter describes the pulse-chase radiolabeling procedure in mice and its applications in detecting axonal transport defects. Two hypotheses—the assembled polymer and the unassembled subunit transport models—have been presented in the chapter to explain the movement of IF proteins into axons. Pulse-labeling experiments with [ 35 S]methionine revealed that components of anterograde transport can be distinguished by both their speed and the cargoes transported; the fast component is the movement of membrane-bound organelles along the microtubules track, while the slow axonal transport conveys cytoskeletal proteins. The slow transport wave observed in vivo by the pulsed radiolabeled techniques is the sum of individual neurofilament (NF) movements spending most of their time pausing. Defects in the axonal transport of NF proteins have been detected with pulse-chase labeling with [ 35 S]methionine in various animal models of pathological conditions. Pulse-chase labeling with [ 35 S]methionine constitutes the standard procedure to analyze the in vivo transport of cytoskeletal proteins down the sciatic nerve or the optic nerve of mice. After the injection of this radioactive amino acid into cell bodies of spinal motor neurons or RGC neurons, the isotope emission allows the monitoring of newly synthesized IF proteins as they travel within axons. From the analysis of the distance covered by radiolabeled proteins at different time intervals, the rate of their transport can be measured." @default.
• W1490346887 created "2016-06-24" @default.
• W1490346887 creator A5072928177 @default.
• W1490346887 creator A5091664460 @default.
• W1490346887 date "2004-01-01" @default.
• W1490346887 modified "2023-10-15" @default.
• W1490346887 title "[35S]Methionine Metabolic Labeling to Study Axonal Transport of Neuronal Intermediate Filament Proteins In Vivo" @default.
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• W1490346887 doi "https://doi.org/10.1016/s0091-679x(04)78019-x" @default.
• W1490346887 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15646631" @default.
• W1490346887 hasPublicationYear "2004" @default.
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