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• W1490348045 endingPage "2210" @default.
• W1490348045 startingPage "2203" @default.
• W1490348045 abstract "The genetic diversity of Desulfovibrio species in environmental samples was determined by denaturing gradient gel electrophoresis (DGGE) of PCR-amplified [NiFe] hydrogenase gene fragments. Five different PCR primers were designed after comparative analysis of [NiFe] hydrogenase gene sequences from three Desulfovibrio species. These primers were tested in different combinations on the genomic DNAs of a variety of hydrogenase-containing and hydrogenase-lacking bacteria. One primer pair was found to be specific for Desulfovibrio species only, while the others gave positive results with other bacteria also. By using this specific primer pair, we were able to amplify the [NiFe] hydrogenase genes of DNAs isolated from environmental samples and to detect the presence of Desulfovibrio species in these samples. However, only after DGGE analysis of these PCR products could the number of different Desulfovibrio species within the samples be determined. DGGE analysis of PCR products from different bioreactors demonstrated up to two bands, while at least five distinguishable bands were detected in a microbial mat sample. Because these bands most likely represent as many Desulfovibrio species present in these samples, we conclude that the genetic diversity of Desulfovibrio species in the natural microbial mat is far greater than that in the experimental bioreactors." @default.
• W1490348045 created "2016-06-24" @default.
• W1490348045 creator A5043873341 @default.
• W1490348045 creator A5082618449 @default.
• W1490348045 date "1995-06-01" @default.
• W1490348045 modified "2023-10-17" @default.
• W1490348045 title "Genetic diversity of Desulfovibrio spp. in environmental samples analyzed by denaturing gradient gel electrophoresis of [NiFe] hydrogenase gene fragments" @default.
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• W1490348045 doi "https://doi.org/10.1128/aem.61.6.2203-2210.1995" @default.
• W1490348045 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/167491" @default.
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