Matches in SemOpenAlex for { <https://semopenalex.org/work/W1490506565> ?p ?o ?g. }
Showing items 1 to 92 of
92
with 100 items per page.
- W1490506565 endingPage "15059" @default.
- W1490506565 startingPage "15054" @default.
- W1490506565 abstract "D-Amino-acid oxidase catalyzes the oxidation of D-amino acids to imino acids. In the oxidative half-reaction, oxygen reacts with the reduced enzyme-imino acid complex to reoxidize the bound FAD. This is then followed by dissociation of the imino acid. The effects of pH and D2O on the kinetics of the oxidative half-reaction of D-amino-acid oxidase have been determined with glycine, D-alanine, and D-serine as substrates. Reaction of the reduced enzyme with oxygen requires that a group with a pKa value of about 10.5 be protonated and a group with a pKa value of 8.5 be deprotonated. The former value is not seen with D-alanine as substrate; the latter is only seen with glycine. No solvent isotope effects are seen on the V/KO2 value with D-alanine, consistent with rate-limiting electron transfer. Product release involves a pH-dependent conformational change. This is rate-limiting at all pH values with D-alanine as substrate. Significant solvent isotope effects are seen on the Vmax value with D-alanine. The proton inventory at high pH is linear, consistent with release of a single proton in the slow step; at pH 6 the solvent inventory is bowl-shaped, consistent with a solvent isotope effect on the conformation of the protein. With glycine the DV value increases to the intrinsic value at pH 10.5; this establishes that CH bond cleavage becomes rate-limiting with this substrate above pH 10." @default.
- W1490506565 created "2016-06-24" @default.
- W1490506565 creator A5053442721 @default.
- W1490506565 creator A5077817714 @default.
- W1490506565 date "1994-05-01" @default.
- W1490506565 modified "2023-09-30" @default.
- W1490506565 title "pH and kinetic isotope effects on the oxidative half-reaction of D-amino-acid oxidase." @default.
- W1490506565 cites W1497254911 @default.
- W1490506565 cites W1499417317 @default.
- W1490506565 cites W1502998518 @default.
- W1490506565 cites W1509599942 @default.
- W1490506565 cites W1510999232 @default.
- W1490506565 cites W1516068362 @default.
- W1490506565 cites W1562598718 @default.
- W1490506565 cites W1649241083 @default.
- W1490506565 cites W1756287317 @default.
- W1490506565 cites W1820160793 @default.
- W1490506565 cites W1970443625 @default.
- W1490506565 cites W1979463607 @default.
- W1490506565 cites W1996811682 @default.
- W1490506565 cites W2008418306 @default.
- W1490506565 cites W2023985286 @default.
- W1490506565 cites W2041331863 @default.
- W1490506565 cites W2085349352 @default.
- W1490506565 cites W2107292115 @default.
- W1490506565 cites W2969273703 @default.
- W1490506565 cites W345718608 @default.
- W1490506565 doi "https://doi.org/10.1016/s0021-9258(17)36573-0" @default.
- W1490506565 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7910822" @default.
- W1490506565 hasPublicationYear "1994" @default.
- W1490506565 type Work @default.
- W1490506565 sameAs 1490506565 @default.
- W1490506565 citedByCount "23" @default.
- W1490506565 countsByYear W14905065652012 @default.
- W1490506565 countsByYear W14905065652013 @default.
- W1490506565 countsByYear W14905065652014 @default.
- W1490506565 countsByYear W14905065652015 @default.
- W1490506565 countsByYear W14905065652016 @default.
- W1490506565 countsByYear W14905065652017 @default.
- W1490506565 countsByYear W14905065652019 @default.
- W1490506565 countsByYear W14905065652022 @default.
- W1490506565 crossrefType "journal-article" @default.
- W1490506565 hasAuthorship W1490506565A5053442721 @default.
- W1490506565 hasAuthorship W1490506565A5077817714 @default.
- W1490506565 hasBestOaLocation W14905065651 @default.
- W1490506565 hasConcept C121332964 @default.
- W1490506565 hasConcept C135889238 @default.
- W1490506565 hasConcept C164304813 @default.
- W1490506565 hasConcept C181199279 @default.
- W1490506565 hasConcept C185592680 @default.
- W1490506565 hasConcept C2778239910 @default.
- W1490506565 hasConcept C38485361 @default.
- W1490506565 hasConcept C515207424 @default.
- W1490506565 hasConcept C55493867 @default.
- W1490506565 hasConcept C57600042 @default.
- W1490506565 hasConcept C58364064 @default.
- W1490506565 hasConcept C62520636 @default.
- W1490506565 hasConcept C69928629 @default.
- W1490506565 hasConceptScore W1490506565C121332964 @default.
- W1490506565 hasConceptScore W1490506565C135889238 @default.
- W1490506565 hasConceptScore W1490506565C164304813 @default.
- W1490506565 hasConceptScore W1490506565C181199279 @default.
- W1490506565 hasConceptScore W1490506565C185592680 @default.
- W1490506565 hasConceptScore W1490506565C2778239910 @default.
- W1490506565 hasConceptScore W1490506565C38485361 @default.
- W1490506565 hasConceptScore W1490506565C515207424 @default.
- W1490506565 hasConceptScore W1490506565C55493867 @default.
- W1490506565 hasConceptScore W1490506565C57600042 @default.
- W1490506565 hasConceptScore W1490506565C58364064 @default.
- W1490506565 hasConceptScore W1490506565C62520636 @default.
- W1490506565 hasConceptScore W1490506565C69928629 @default.
- W1490506565 hasIssue "21" @default.
- W1490506565 hasLocation W14905065651 @default.
- W1490506565 hasOpenAccess W1490506565 @default.
- W1490506565 hasPrimaryLocation W14905065651 @default.
- W1490506565 hasRelatedWork W1513383154 @default.
- W1490506565 hasRelatedWork W1932532950 @default.
- W1490506565 hasRelatedWork W2023130986 @default.
- W1490506565 hasRelatedWork W2023307655 @default.
- W1490506565 hasRelatedWork W2033847485 @default.
- W1490506565 hasRelatedWork W2046498090 @default.
- W1490506565 hasRelatedWork W2051826279 @default.
- W1490506565 hasRelatedWork W2073375101 @default.
- W1490506565 hasRelatedWork W2162592118 @default.
- W1490506565 hasRelatedWork W2900504840 @default.
- W1490506565 hasVolume "269" @default.
- W1490506565 isParatext "false" @default.
- W1490506565 isRetracted "false" @default.
- W1490506565 magId "1490506565" @default.
- W1490506565 workType "article" @default.