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• W1490687307 abstract "We have investigated whether the phosphorylation of the Mr = 35,000 subunit of eukaryotic initiation factor 2 (eIF-2 alpha) by the hemin-controlled translational repressor (HCR), which appears to mediate the regulation of protein synthesis by hemin in rabbit reticulocytes, occurs at a single or multiple sites. Following phosphorylation of highly purified ribosomal or soluble eIF-2 with [gamma-32P]ATP and highly purified HCR, eIF-2 alpha was isolated by elution from a 7% sodium dodecyl sulfate-polyacrylamide gel, subjected to trypsin digestion, and analyzed on 20% sodium dodecyl sulfate-polyacrylamide gels. By 9 h of digestion none of the original, Mr = 35,000 subunit remained, and after 24 h the 32P radioactivity was distributed in multiple polypeptides in the range of Mr = 4,000 to 8,000. After 4 days of trypsinization, however, all of these peptides were reduced to a low level except for a minor band with Mr = 4,500, and a Mr = 1,500 component became the predominant 32P-labeled product. After 6 days of trypsin digestion the minor Mr = 4,500 component was much less but still detectable, and the Mr = 1,500 component represented 85% (ribosomal eIF-2 alpha) and 90% (soluble eIF-2 alpha) of the total radioactivity. The Mr = 1,500 component, when eluted fro 20% gels, showed a single spot when fingerprinted on thin layer cellulose sheets that was identical whether derived from ribosomal or soluble eIF-2 alpha. These findings suggest that HCR phosphorylates eIF-2 alpha at one specific site, although we cannot exclude the possibility that a second, minor site may also become phosphorylated to a very limited degree." @default.
• W1490687307 created "2016-06-24" @default.
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• W1490687307 date "1981-01-01" @default.
• W1490687307 modified "2023-10-18" @default.
• W1490687307 title "Evidence that the phosphorylation of eukaryotic initiation factor 2 alpha by the hemin-controlled translational repressor occurs at a single site." @default.
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• W1490687307 doi "https://doi.org/10.1016/s0021-9258(19)70013-1" @default.
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