Matches in SemOpenAlex for { <https://semopenalex.org/work/W1490803640> ?p ?o ?g. }
- W1490803640 endingPage "12248" @default.
- W1490803640 startingPage "12242" @default.
- W1490803640 abstract "Abstract gp115 is a N- and O-glycosylated protein of Saccharomyces cerevisiae. It is also modified by addition of glycosylphosphatidylinositol, which anchors the protein to the plasma membrane. The gene encoding gp115 (GGP1) has been cloned by a two-step procedure. By an immunoscreening of a yeast genomic DNA library the expression vector lambda gt11, a 3'-terminal 0.9-kilobase portion of the gene has been isolated and then used as a molecular probe to screen a yeast genomic DNA library YEp24. In this way, the whole GGP1 gene has been cloned. Its identity with the gp115 gene has been confirmed by gene disruption, which has also indicated that the function of gp115 is not essential for cell viability. The features of the sequence are also entirely consistent with it corresponding to the gp115 gene. The nucleotide sequence of GGP1 predicts a 60-kDa polypeptide, agreement with the molecular mass of the gp115 precursor detected sec53 mutant cells at restrictive temperature. Two hydrophobic sequences, one NH2- and the other COOH-terminal were found. The former has the features of the cleavable signal sequence, which allows the entry of proteins the secretory pathway. The latter could be the signal sequence that has to be removed during the addition of glycosylphosphatidylinositol. The predicted amino acid sequence of gp115 shows 10 sequons for N-glycosylation and a high proportion of serine-threonine residues (22%) that could provide several sites for O-glycosylation. The unusual concentration of 27 serines the COOH-terminal portion of the protein shares homology with a similar polyserine repeat of the serine repeat antigen (SERA protein) of Plasmodium falciparum. A two-dimensional analysis of the in vitro translational product of the GGP1 mRNA has been carried out, allowing the identification of the in vivo gp115 precursor a two-dimensional gel." @default.
- W1490803640 created "2016-06-24" @default.
- W1490803640 creator A5047736377 @default.
- W1490803640 creator A5053427006 @default.
- W1490803640 creator A5054077550 @default.
- W1490803640 creator A5081015298 @default.
- W1490803640 creator A5091051421 @default.
- W1490803640 date "1991-07-01" @default.
- W1490803640 modified "2023-10-18" @default.
- W1490803640 title "Isolation and deduced amino acid sequence of the gene encoding gp115, a yeast glycophospholipid-anchored protein containing a serine-rich region" @default.
- W1490803640 cites W1499378420 @default.
- W1490803640 cites W1513847907 @default.
- W1490803640 cites W1533484029 @default.
- W1490803640 cites W1537782690 @default.
- W1490803640 cites W1555564714 @default.
- W1490803640 cites W158802860 @default.
- W1490803640 cites W1606423922 @default.
- W1490803640 cites W1910203533 @default.
- W1490803640 cites W1919855132 @default.
- W1490803640 cites W1969859852 @default.
- W1490803640 cites W1973782344 @default.
- W1490803640 cites W1975304761 @default.
- W1490803640 cites W1985488253 @default.
- W1490803640 cites W2003676968 @default.
- W1490803640 cites W2006238316 @default.
- W1490803640 cites W2015153859 @default.
- W1490803640 cites W2020733135 @default.
- W1490803640 cites W2024194876 @default.
- W1490803640 cites W2026582002 @default.
- W1490803640 cites W2031847747 @default.
- W1490803640 cites W2032462421 @default.
- W1490803640 cites W2033518938 @default.
- W1490803640 cites W2050611086 @default.
- W1490803640 cites W2051043527 @default.
- W1490803640 cites W2056530947 @default.
- W1490803640 cites W2060096281 @default.
- W1490803640 cites W2064864270 @default.
- W1490803640 cites W2065024200 @default.
- W1490803640 cites W2069573006 @default.
- W1490803640 cites W2069705950 @default.
- W1490803640 cites W2071477986 @default.
- W1490803640 cites W2073351370 @default.
- W1490803640 cites W2074939600 @default.
- W1490803640 cites W2078119356 @default.
- W1490803640 cites W2080795403 @default.
- W1490803640 cites W2081499960 @default.
- W1490803640 cites W2082156530 @default.
- W1490803640 cites W2082775739 @default.
- W1490803640 cites W2094195202 @default.
- W1490803640 cites W2100837269 @default.
- W1490803640 cites W2101108802 @default.
- W1490803640 cites W2134887033 @default.
- W1490803640 cites W2138270253 @default.
- W1490803640 cites W2138879032 @default.
- W1490803640 cites W2168370663 @default.
- W1490803640 cites W2171885622 @default.
- W1490803640 cites W2296812489 @default.
- W1490803640 doi "https://doi.org/10.1016/s0021-9258(18)98888-5" @default.
- W1490803640 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2061310" @default.
- W1490803640 hasPublicationYear "1991" @default.
- W1490803640 type Work @default.
- W1490803640 sameAs 1490803640 @default.
- W1490803640 citedByCount "81" @default.
- W1490803640 countsByYear W14908036402013 @default.
- W1490803640 countsByYear W14908036402016 @default.
- W1490803640 crossrefType "journal-article" @default.
- W1490803640 hasAuthorship W1490803640A5047736377 @default.
- W1490803640 hasAuthorship W1490803640A5053427006 @default.
- W1490803640 hasAuthorship W1490803640A5054077550 @default.
- W1490803640 hasAuthorship W1490803640A5081015298 @default.
- W1490803640 hasAuthorship W1490803640A5091051421 @default.
- W1490803640 hasBestOaLocation W14908036401 @default.
- W1490803640 hasConcept C104317684 @default.
- W1490803640 hasConcept C125411270 @default.
- W1490803640 hasConcept C167625842 @default.
- W1490803640 hasConcept C169760540 @default.
- W1490803640 hasConcept C181199279 @default.
- W1490803640 hasConcept C185592680 @default.
- W1490803640 hasConcept C2775941552 @default.
- W1490803640 hasConcept C2776414213 @default.
- W1490803640 hasConcept C2778112365 @default.
- W1490803640 hasConcept C2779222958 @default.
- W1490803640 hasConcept C515207424 @default.
- W1490803640 hasConcept C55493867 @default.
- W1490803640 hasConcept C60644358 @default.
- W1490803640 hasConcept C86803240 @default.
- W1490803640 hasConceptScore W1490803640C104317684 @default.
- W1490803640 hasConceptScore W1490803640C125411270 @default.
- W1490803640 hasConceptScore W1490803640C167625842 @default.
- W1490803640 hasConceptScore W1490803640C169760540 @default.
- W1490803640 hasConceptScore W1490803640C181199279 @default.
- W1490803640 hasConceptScore W1490803640C185592680 @default.
- W1490803640 hasConceptScore W1490803640C2775941552 @default.
- W1490803640 hasConceptScore W1490803640C2776414213 @default.
- W1490803640 hasConceptScore W1490803640C2778112365 @default.
- W1490803640 hasConceptScore W1490803640C2779222958 @default.
- W1490803640 hasConceptScore W1490803640C515207424 @default.
- W1490803640 hasConceptScore W1490803640C55493867 @default.