FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1490988370> ?p ?o ?g. }

• W1490988370 endingPage "14863" @default.
• W1490988370 startingPage "14858" @default.
• W1490988370 abstract "The effect of the tumor promoter, 12-O-tetradecanoylphorbol-13-acetate (TPA), on phospholipid degradation was investigated in three cell lines of dissimilar origin, Madin-Darby canine kidney cells (MDCK), rat aorta smooth muscle cells (RASM), and bovine pulmonary artery endothelial cells (BPAE). In cells prelabeled with [3H]myristic acid, which is predominantly incorporated into phosphatidylcholine (PC), TPA treatment (80 nM) in the absence or presence of ethanol (2%) in the culture medium resulted in either the rapid generation of [3H]phosphatidate (PA) or the sustained accumulation of [3H]phosphatidylethanol (PEt), respectively. Increases in [3H]PA and [3H]PEt were paralleled by quantitative decreases in cellular [3H]PC radioactivity. TPA-induced [3H]PEt formation occurred in a similar fashion, irrespective of the presence of Ca2+ in the culture medium. The experiments demonstrate that TPA elicits PC degradation by phospholipase D (PLD) in cells of diverse origin. Data from further experiments revealed a complex relationship between TPA-induced [3H]PA and [3H]diacylglycerol (DG) generation in the three cell lines that was suggestive of dual pathways for the generation of [3H]DG. Experiments to discern the pathways for TPA-induced, PC-derived DG were conducted by comparing the variation of [3H]PA and [3H]DG formation in the absence and in the presence of increasing ethanol concentrations in the culture medium. With increasing amounts of ethanol, the formation of [3H]PA decreased at the expense of [3H]PEt formation, and depending upon the pathway operable, the amount of [3H]DG formed was either decreased, indicative of indirect formation of DG via PA phosphohydrolase, or not modified, indicative of DG formation by a direct phospholipase C (PLC) pathway. Increasing the concentration of ethanol in the medium blocked TPA-induced [3H]DG generation in MDCK cells in a concentration-dependent manner, while the formation of [3H]PEt increased at the expense of [3H]PA formation. In BPAE cells the presence of ethanol likewise reduced TPA-elicited formation of DG. Conversely, in two smooth muscle cell lines, RASM and A-10, ethanol was without influence on TPA-induced formation of [3H]DG, although [3H]PEt was generated at the expense of [3H]PA. In RASM cells prelabeled with [3H]choline, TPA induced the release to the medium of [3H]choline and [3H]phosphocholine, indicative of both PLD and PLC activation. These results show that TPA elicits DG formation from PC in MDCK cells predominantly by an indirect pathway, whereas in arterial smooth muscle cells DG is formed in part by the direct action of PLC.(ABSTRACT TRUNCATED AT 400 WORDS)" @default.
• W1490988370 created "2016-06-24" @default.
• W1490988370 creator A5031949915 @default.
• W1490988370 creator A5084512743 @default.
• W1490988370 date "1990-09-01" @default.
• W1490988370 modified "2023-10-15" @default.
• W1490988370 title "Phorbol diesters stimulate the accumulation of phosphatidate, phosphatidylethanol, and diacylglycerol in three cell types. Evidence for the indirect formation of phosphatidylcholine-derived diacylglycerol by a phospholipase D pathway and direct formation of diacylglycerol by a phospholipase C pathway." @default.
• W1490988370 cites W1493283928 @default.
• W1490988370 cites W1511600524 @default.
• W1490988370 cites W1518259698 @default.
• W1490988370 cites W1528198451 @default.
• W1490988370 cites W1560104562 @default.
• W1490988370 cites W1564094331 @default.
• W1490988370 cites W1573008064 @default.
• W1490988370 cites W1578978258 @default.
• W1490988370 cites W1580927583 @default.
• W1490988370 cites W1605324556 @default.
• W1490988370 cites W162053295 @default.
• W1490988370 cites W1695689747 @default.
• W1490988370 cites W1973153969 @default.
• W1490988370 cites W1998490733 @default.
• W1490988370 cites W2001588855 @default.
• W1490988370 cites W2006031540 @default.
• W1490988370 cites W2014555925 @default.
• W1490988370 cites W2015546115 @default.
• W1490988370 cites W2017733935 @default.
• W1490988370 cites W2025092789 @default.
• W1490988370 cites W2031577443 @default.
• W1490988370 cites W2043570857 @default.
• W1490988370 cites W2044508076 @default.
• W1490988370 cites W2046489172 @default.
• W1490988370 cites W2059897580 @default.
• W1490988370 cites W2061916449 @default.
• W1490988370 cites W2068877244 @default.
• W1490988370 cites W2073873542 @default.
• W1490988370 cites W2077547402 @default.
• W1490988370 cites W2111691978 @default.
• W1490988370 cites W2259083375 @default.
• W1490988370 cites W2300552860 @default.
• W1490988370 cites W2317176566 @default.
• W1490988370 cites W2328589028 @default.
• W1490988370 doi "https://doi.org/10.1016/s0021-9258(18)77193-7" @default.
• W1490988370 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2394701" @default.
• W1490988370 hasPublicationYear "1990" @default.
• W1490988370 type Work @default.
• W1490988370 sameAs 1490988370 @default.
• W1490988370 citedByCount "114" @default.
• W1490988370 countsByYear W14909883702013 @default.
• W1490988370 countsByYear W14909883702015 @default.
• W1490988370 countsByYear W14909883702016 @default.
• W1490988370 countsByYear W14909883702022 @default.
• W1490988370 crossrefType "journal-article" @default.
• W1490988370 hasAuthorship W1490988370A5031949915 @default.
• W1490988370 hasAuthorship W1490988370A5084512743 @default.
• W1490988370 hasBestOaLocation W14909883701 @default.
• W1490988370 hasConcept C181199279 @default.
• W1490988370 hasConcept C185592680 @default.
• W1490988370 hasConcept C195794163 @default.
• W1490988370 hasConcept C2776330855 @default.
• W1490988370 hasConcept C2778918659 @default.
• W1490988370 hasConcept C2780202252 @default.
• W1490988370 hasConcept C2909482567 @default.
• W1490988370 hasConcept C36020004 @default.
• W1490988370 hasConcept C41625074 @default.
• W1490988370 hasConcept C55493867 @default.
• W1490988370 hasConcept C6182249 @default.
• W1490988370 hasConcept C86803240 @default.
• W1490988370 hasConcept C95444343 @default.
• W1490988370 hasConceptScore W1490988370C181199279 @default.
• W1490988370 hasConceptScore W1490988370C185592680 @default.
• W1490988370 hasConceptScore W1490988370C195794163 @default.
• W1490988370 hasConceptScore W1490988370C2776330855 @default.
• W1490988370 hasConceptScore W1490988370C2778918659 @default.
• W1490988370 hasConceptScore W1490988370C2780202252 @default.
• W1490988370 hasConceptScore W1490988370C2909482567 @default.
• W1490988370 hasConceptScore W1490988370C36020004 @default.
• W1490988370 hasConceptScore W1490988370C41625074 @default.
• W1490988370 hasConceptScore W1490988370C55493867 @default.
• W1490988370 hasConceptScore W1490988370C6182249 @default.
• W1490988370 hasConceptScore W1490988370C86803240 @default.
• W1490988370 hasConceptScore W1490988370C95444343 @default.
• W1490988370 hasIssue "25" @default.
• W1490988370 hasLocation W14909883701 @default.
• W1490988370 hasOpenAccess W1490988370 @default.
• W1490988370 hasPrimaryLocation W14909883701 @default.
• W1490988370 hasRelatedWork W1490988370 @default.
• W1490988370 hasRelatedWork W1967921776 @default.
• W1490988370 hasRelatedWork W1970471130 @default.
• W1490988370 hasRelatedWork W1989864251 @default.
• W1490988370 hasRelatedWork W1995078152 @default.
• W1490988370 hasRelatedWork W2015546115 @default.
• W1490988370 hasRelatedWork W2095093323 @default.
• W1490988370 hasRelatedWork W766120566 @default.
• W1490988370 hasRelatedWork W803639510 @default.
• W1490988370 hasRelatedWork W2467068113 @default.
• W1490988370 hasVolume "265" @default.
• W1490988370 isParatext "false" @default.
• W1490988370 isRetracted "false" @default.

• next