Matches in SemOpenAlex for { <https://semopenalex.org/work/W1490993384> ?p ?o ?g. }
Showing items 1 to 93 of
93
with 100 items per page.
- W1490993384 endingPage "311" @default.
- W1490993384 startingPage "303" @default.
- W1490993384 abstract "An optimized in vitro assay of 3'-phosphoadenylysulfate:galactosylceramide 3'-sulfotransferase (EC 2.8.2.11, galactosylceramide sulfotransferase, formerly known as galactocerebroside sulfotransferase) activity is presented, that can be used in crude homogenate of brain tissue of various developmental stages. The enzyme activity is determined by measuring the [35S]sulfatides formed by the enzymic transfer of [35S]sulfate from 3'-phosphoadenoside 5'-phospho [35S]sulfate to galactosylceramides. The sulfatide formation at 30 degrees C is linear up to 30 min and up to a protein concentration of 1 mg per 0.5 ml assay volume. The presence of 0.4% Triton X-100 and 50 micrometer exogenous bovine cerebrosides are optimal for enzyme activity. The pH optimum of the reaction is at pH 6.5 using 0.1 M imidazole buffer. The enzyme reaction is stimulated by NaCl, KCl, MgCl2, CaCl2, MnCl2, ATP and inhibited by ADP. The developmental enzyme activity pattern of mouse brain is the same, if derived from homogenates and microsomes, respectively, under our assay conditions." @default.
- W1490993384 created "2016-06-24" @default.
- W1490993384 creator A5007448833 @default.
- W1490993384 creator A5009384694 @default.
- W1490993384 creator A5025826885 @default.
- W1490993384 creator A5030722744 @default.
- W1490993384 date "1977-08-01" @default.
- W1490993384 modified "2023-09-26" @default.
- W1490993384 title "3′-Phosphoadenylylsulfate:galactosylceramide 3′-sulfotransferase" @default.
- W1490993384 cites W111867139 @default.
- W1490993384 cites W1498577073 @default.
- W1490993384 cites W1775749144 @default.
- W1490993384 cites W1900191146 @default.
- W1490993384 cites W1969417541 @default.
- W1490993384 cites W2004415435 @default.
- W1490993384 cites W2014219194 @default.
- W1490993384 cites W2035320930 @default.
- W1490993384 cites W2068412008 @default.
- W1490993384 cites W2068950953 @default.
- W1490993384 cites W2071179144 @default.
- W1490993384 cites W2079459910 @default.
- W1490993384 cites W2087624306 @default.
- W1490993384 cites W2090291124 @default.
- W1490993384 cites W2104749442 @default.
- W1490993384 cites W2105205362 @default.
- W1490993384 cites W2168526937 @default.
- W1490993384 doi "https://doi.org/10.1016/0005-2744(77)90058-4" @default.
- W1490993384 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19067" @default.
- W1490993384 hasPublicationYear "1977" @default.
- W1490993384 type Work @default.
- W1490993384 sameAs 1490993384 @default.
- W1490993384 citedByCount "24" @default.
- W1490993384 crossrefType "journal-article" @default.
- W1490993384 hasAuthorship W1490993384A5007448833 @default.
- W1490993384 hasAuthorship W1490993384A5009384694 @default.
- W1490993384 hasAuthorship W1490993384A5025826885 @default.
- W1490993384 hasAuthorship W1490993384A5030722744 @default.
- W1490993384 hasConcept C155138218 @default.
- W1490993384 hasConcept C169760540 @default.
- W1490993384 hasConcept C178790620 @default.
- W1490993384 hasConcept C181199279 @default.
- W1490993384 hasConcept C185592680 @default.
- W1490993384 hasConcept C202751555 @default.
- W1490993384 hasConcept C2775904581 @default.
- W1490993384 hasConcept C2776985911 @default.
- W1490993384 hasConcept C2778343803 @default.
- W1490993384 hasConcept C2778609137 @default.
- W1490993384 hasConcept C2781017270 @default.
- W1490993384 hasConcept C28212737 @default.
- W1490993384 hasConcept C43617362 @default.
- W1490993384 hasConcept C529278444 @default.
- W1490993384 hasConcept C55493867 @default.
- W1490993384 hasConcept C86803240 @default.
- W1490993384 hasConcept C87644729 @default.
- W1490993384 hasConceptScore W1490993384C155138218 @default.
- W1490993384 hasConceptScore W1490993384C169760540 @default.
- W1490993384 hasConceptScore W1490993384C178790620 @default.
- W1490993384 hasConceptScore W1490993384C181199279 @default.
- W1490993384 hasConceptScore W1490993384C185592680 @default.
- W1490993384 hasConceptScore W1490993384C202751555 @default.
- W1490993384 hasConceptScore W1490993384C2775904581 @default.
- W1490993384 hasConceptScore W1490993384C2776985911 @default.
- W1490993384 hasConceptScore W1490993384C2778343803 @default.
- W1490993384 hasConceptScore W1490993384C2778609137 @default.
- W1490993384 hasConceptScore W1490993384C2781017270 @default.
- W1490993384 hasConceptScore W1490993384C28212737 @default.
- W1490993384 hasConceptScore W1490993384C43617362 @default.
- W1490993384 hasConceptScore W1490993384C529278444 @default.
- W1490993384 hasConceptScore W1490993384C55493867 @default.
- W1490993384 hasConceptScore W1490993384C86803240 @default.
- W1490993384 hasConceptScore W1490993384C87644729 @default.
- W1490993384 hasIssue "2" @default.
- W1490993384 hasLocation W14909933841 @default.
- W1490993384 hasLocation W14909933842 @default.
- W1490993384 hasOpenAccess W1490993384 @default.
- W1490993384 hasPrimaryLocation W14909933841 @default.
- W1490993384 hasRelatedWork W1975340112 @default.
- W1490993384 hasRelatedWork W2000259043 @default.
- W1490993384 hasRelatedWork W2000309406 @default.
- W1490993384 hasRelatedWork W2003094270 @default.
- W1490993384 hasRelatedWork W2010372900 @default.
- W1490993384 hasRelatedWork W2016514135 @default.
- W1490993384 hasRelatedWork W2042153386 @default.
- W1490993384 hasRelatedWork W2042354212 @default.
- W1490993384 hasRelatedWork W2147593455 @default.
- W1490993384 hasRelatedWork W3165496361 @default.
- W1490993384 hasVolume "483" @default.
- W1490993384 isParatext "false" @default.
- W1490993384 isRetracted "false" @default.
- W1490993384 magId "1490993384" @default.
- W1490993384 workType "article" @default.