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- W1491108152 abstract "An automation-assisted method enables DNA extraction of over a thousand yeast colonies in a day by one person. Yeast DNA is extracted essentially quantitatively in three steps: (a) cells are converted into spheroplasts with yeast lytic enzyme, (b) cells are lysed with proteinase K, and (c) DNA is collected by ethanol precipitation. The DNA stored at 4 degrees C is active in polymerase chain reaction experiments for more than a year." @default.
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- W1491108152 date "1994-01-01" @default.
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- W1491108152 title "High-throughput rapid yeast DNA extraction" @default.
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- W1491108152 doi "https://doi.org/10.1016/1050-3862(94)90003-5" @default.
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