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- W1491165482 abstract "4468 Objectives: Seven salivary oral cancer transcriptome biomarkers (SOCTB: SAT1, S100P, OAZ1, IL1B, IL8, H3F3A and DUSP1) have been identified to distinguish oral cancer from normal controls by relative quantification. However, technological variables inherent in DNA-based standard quantitative RT-PCR, as well as differential RNA degradation and RNA input created baseline shifts that prevented use of a cut-off threshold between experiments to segregate oral cancer from controls. The purpose of this study is to further validate the 7 biomarkers and to establish a genuine absolute quantitative PCR using RNA-based standard quantification, and using saliva internal control gene to normalize input RNA quantity, to make salivary RNA biomarker measurement more accurate and reproducible and solve the base-line shifting problem. Methods: For detecting each of the SOCTB, technology variance was explored by utilizing genuine absolute quantification. Primers for specific amplification of each of 7 SOCTB mRNAs with T 7 promoter sequence fused to 5’ end were designed and used to generate recombinant RNA(recRNA). Quantitation of recRNA was accurately measured with Nanodrop bioanalyser, and serial dilution was used to generate standard. Each set of standard was quantified by real time quantitativePCR (qPCR) parallel with saliva RNA samples. RNA input and degradation was explored by normalization to saliva internal reference (SIR) genes. WilCOXon test and ROC analysis were used to judge the quality of makers. Results: qPCR data has shown that in 4 independent detection trials, the 7 SOCTB gene expression levels are significantly elevated (> 1.5 fold, p" @default.
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- W1491165482 date "2006-04-15" @default.
- W1491165482 modified "2023-09-24" @default.
- W1491165482 title "Salivary oral cancer transcriptome biomarkers (SOCTB) for clinical detection" @default.
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