Matches in SemOpenAlex for { <https://semopenalex.org/work/W1491300294> ?p ?o ?g. }
Showing items 1 to 81 of
81
with 100 items per page.
- W1491300294 abstract "Two-dimensional gel electrophoresis (2D-PAGE) has proven over the years to be a reliable and efficient method for separation of hundreds of proteins based on charge and mass. Nevertheless, the complexity of even the simplest proteomes limits the resolving power of 2D-PAGE. This limitation can be partially alleviated by sample prefractionation using a variety of techniques. Here, we have used Vivapure Ion Exchange centrifugal adsorber units to rapidly prefractionate total fission yeast protein lysate based on protein charge. Three fractions were prepared by stepwise elution with increasing sodium chloride concentrations. Each of the fractions, as well as the total lysate, were analyzed by 2D-PAGE. This simple prefractionation procedure considerably increased the resolving power of 2D-PAGE. Whereas 308 spots could be detected by analysing total protein lysate, 910 spots were observed upon prefractionation. Thorough gel image analysis demonstrated that prefractionation visualizes an additional set of 458 unique fission yeast proteins not detected in whole cell lysate. Prefractionation with Vivapure Q spin columns proved to be a simple, fast, reproducible, and cost-effective means of increasing the resolving power of 2D-PAGE using standard laboratory equipment." @default.
- W1491300294 created "2016-06-24" @default.
- W1491300294 creator A5014564580 @default.
- W1491300294 creator A5032025654 @default.
- W1491300294 creator A5034052307 @default.
- W1491300294 creator A5055618922 @default.
- W1491300294 creator A5060703811 @default.
- W1491300294 creator A5062880636 @default.
- W1491300294 creator A5076883302 @default.
- W1491300294 creator A5086113043 @default.
- W1491300294 date "2004-04-26" @default.
- W1491300294 modified "2023-10-17" @default.
- W1491300294 title "Rapid prefractionation of complex protein lysates with centrifugal membrane adsorber units improves the resolving power of 2D-PAGE-based proteome analysis" @default.
- W1491300294 cites W1606423922 @default.
- W1491300294 cites W1986052465 @default.
- W1491300294 cites W2013947447 @default.
- W1491300294 cites W2396773613 @default.
- W1491300294 doi "https://doi.org/10.1186/1471-2164-5-25" @default.
- W1491300294 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/406497" @default.
- W1491300294 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15109393" @default.
- W1491300294 hasPublicationYear "2004" @default.
- W1491300294 type Work @default.
- W1491300294 sameAs 1491300294 @default.
- W1491300294 citedByCount "11" @default.
- W1491300294 countsByYear W14913002942012 @default.
- W1491300294 countsByYear W14913002942014 @default.
- W1491300294 countsByYear W14913002942018 @default.
- W1491300294 countsByYear W14913002942019 @default.
- W1491300294 countsByYear W14913002942021 @default.
- W1491300294 crossrefType "journal-article" @default.
- W1491300294 hasAuthorship W1491300294A5014564580 @default.
- W1491300294 hasAuthorship W1491300294A5032025654 @default.
- W1491300294 hasAuthorship W1491300294A5034052307 @default.
- W1491300294 hasAuthorship W1491300294A5055618922 @default.
- W1491300294 hasAuthorship W1491300294A5060703811 @default.
- W1491300294 hasAuthorship W1491300294A5062880636 @default.
- W1491300294 hasAuthorship W1491300294A5076883302 @default.
- W1491300294 hasAuthorship W1491300294A5086113043 @default.
- W1491300294 hasBestOaLocation W14913002941 @default.
- W1491300294 hasConcept C104397665 @default.
- W1491300294 hasConcept C113196181 @default.
- W1491300294 hasConcept C116084860 @default.
- W1491300294 hasConcept C185592680 @default.
- W1491300294 hasConcept C40250595 @default.
- W1491300294 hasConcept C43617362 @default.
- W1491300294 hasConcept C55493867 @default.
- W1491300294 hasConcept C57409179 @default.
- W1491300294 hasConcept C73826308 @default.
- W1491300294 hasConcept C86803240 @default.
- W1491300294 hasConceptScore W1491300294C104397665 @default.
- W1491300294 hasConceptScore W1491300294C113196181 @default.
- W1491300294 hasConceptScore W1491300294C116084860 @default.
- W1491300294 hasConceptScore W1491300294C185592680 @default.
- W1491300294 hasConceptScore W1491300294C40250595 @default.
- W1491300294 hasConceptScore W1491300294C43617362 @default.
- W1491300294 hasConceptScore W1491300294C55493867 @default.
- W1491300294 hasConceptScore W1491300294C57409179 @default.
- W1491300294 hasConceptScore W1491300294C73826308 @default.
- W1491300294 hasConceptScore W1491300294C86803240 @default.
- W1491300294 hasIssue "1" @default.
- W1491300294 hasLocation W14913002941 @default.
- W1491300294 hasLocation W14913002942 @default.
- W1491300294 hasLocation W14913002943 @default.
- W1491300294 hasLocation W14913002944 @default.
- W1491300294 hasOpenAccess W1491300294 @default.
- W1491300294 hasPrimaryLocation W14913002941 @default.
- W1491300294 hasRelatedWork W1532337558 @default.
- W1491300294 hasRelatedWork W1977017918 @default.
- W1491300294 hasRelatedWork W2004493637 @default.
- W1491300294 hasRelatedWork W2035449592 @default.
- W1491300294 hasRelatedWork W2049373341 @default.
- W1491300294 hasRelatedWork W2050407748 @default.
- W1491300294 hasRelatedWork W2053103690 @default.
- W1491300294 hasRelatedWork W2075361777 @default.
- W1491300294 hasRelatedWork W3157018711 @default.
- W1491300294 hasRelatedWork W1968838065 @default.
- W1491300294 hasVolume "5" @default.
- W1491300294 isParatext "false" @default.
- W1491300294 isRetracted "false" @default.
- W1491300294 magId "1491300294" @default.
- W1491300294 workType "article" @default.