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- W1491488493 abstract "Neurospora crassa mutants deficient in asparagine synthetase were selected by using the procedure of inositol-less death. Complementation tests among the 100 mutants isolated suggested that their alterations were genetically allelic. Recombination analysis with strain S1007t, an asparagine auxotroph, indicated that the mutations were located near or within the asn gene on linkage group V. In vitro assays with a heterokaryon indicated that the mutation was dominant. Thermal instability of cell extracts from temperature-sensitive strains in an in vitro asparagine synthetase assay determined that the mutations were in the structural gene(s) for asparagine synthetase." @default.
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- W1491488493 date "1983-10-01" @default.
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- W1491488493 title "Neurospora crassa mutants deficient in asparagine synthetase" @default.
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- W1491488493 doi "https://doi.org/10.1128/jb.156.1.475-478.1983" @default.
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