Matches in SemOpenAlex for { <https://semopenalex.org/work/W1491670324> ?p ?o ?g. }
Showing items 1 to 92 of
92
with 100 items per page.
- W1491670324 endingPage "28526" @default.
- W1491670324 startingPage "28523" @default.
- W1491670324 abstract "A somatic cell mutant of the Chinese hamster ovary (CHO)-K1 (called Mev-1), auxotrophic for mevalonate by virtue of a complete lack of detectable cytosolic 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) synthase activity, was transfected with a plasmid containing the cDNA for ketogenic mitochondrial HMG-CoA synthase under the control of SV40 early promoter. The resulting stable cell line (Mev-SM) was able to grow in the absence of mevalonate. Analysis of Western blot showed that the new cell line strongly expressed mitochondrial HMG-CoA synthase protein. Immunocytochemical studies using specific antibodies against mitochondrial HMG-CoA synthase showed that the protein was located exclusively inside the mitochondria. The prototroph cell line Mev-SM can incorporate labeled acetate into cholesterol in the absence of mevalonate. These results show that the new cell line may circumvent the lack of cytosolic HMG-CoA synthase activity by producing cholesterol-convertible HMG-CoA inside the mitochondria." @default.
- W1491670324 created "2016-06-24" @default.
- W1491670324 creator A5034363243 @default.
- W1491670324 creator A5036428126 @default.
- W1491670324 creator A5050769125 @default.
- W1491670324 creator A5054502784 @default.
- W1491670324 creator A5057773886 @default.
- W1491670324 creator A5062126745 @default.
- W1491670324 date "1994-11-01" @default.
- W1491670324 modified "2023-10-03" @default.
- W1491670324 title "Transfection of the ketogenic mitochondrial 3-hydroxy-3-methylglutaryl-coenzyme A synthase cDNA into Mev-1 cells corrects their auxotrophy for mevalonate." @default.
- W1491670324 cites W1495163505 @default.
- W1491670324 cites W1514061196 @default.
- W1491670324 cites W1534835840 @default.
- W1491670324 cites W1547196935 @default.
- W1491670324 cites W1686484230 @default.
- W1491670324 cites W1818012174 @default.
- W1491670324 cites W1866494357 @default.
- W1491670324 cites W1875092895 @default.
- W1491670324 cites W1966661789 @default.
- W1491670324 cites W1993325037 @default.
- W1491670324 cites W1998861020 @default.
- W1491670324 cites W2004134440 @default.
- W1491670324 cites W2022163690 @default.
- W1491670324 cites W2059380351 @default.
- W1491670324 cites W2088050113 @default.
- W1491670324 cites W2340675198 @default.
- W1491670324 doi "https://doi.org/10.1016/s0021-9258(19)61932-0" @default.
- W1491670324 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7961793" @default.
- W1491670324 hasPublicationYear "1994" @default.
- W1491670324 type Work @default.
- W1491670324 sameAs 1491670324 @default.
- W1491670324 citedByCount "14" @default.
- W1491670324 countsByYear W14916703242013 @default.
- W1491670324 countsByYear W14916703242016 @default.
- W1491670324 countsByYear W14916703242019 @default.
- W1491670324 countsByYear W14916703242021 @default.
- W1491670324 crossrefType "journal-article" @default.
- W1491670324 hasAuthorship W1491670324A5034363243 @default.
- W1491670324 hasAuthorship W1491670324A5036428126 @default.
- W1491670324 hasAuthorship W1491670324A5050769125 @default.
- W1491670324 hasAuthorship W1491670324A5054502784 @default.
- W1491670324 hasAuthorship W1491670324A5057773886 @default.
- W1491670324 hasAuthorship W1491670324A5062126745 @default.
- W1491670324 hasBestOaLocation W14916703241 @default.
- W1491670324 hasConcept C104317684 @default.
- W1491670324 hasConcept C112243037 @default.
- W1491670324 hasConcept C134651460 @default.
- W1491670324 hasConcept C153911025 @default.
- W1491670324 hasConcept C181199279 @default.
- W1491670324 hasConcept C185592680 @default.
- W1491670324 hasConcept C187882448 @default.
- W1491670324 hasConcept C20950039 @default.
- W1491670324 hasConcept C2779123261 @default.
- W1491670324 hasConcept C54009773 @default.
- W1491670324 hasConcept C547475151 @default.
- W1491670324 hasConcept C55493867 @default.
- W1491670324 hasConcept C86803240 @default.
- W1491670324 hasConceptScore W1491670324C104317684 @default.
- W1491670324 hasConceptScore W1491670324C112243037 @default.
- W1491670324 hasConceptScore W1491670324C134651460 @default.
- W1491670324 hasConceptScore W1491670324C153911025 @default.
- W1491670324 hasConceptScore W1491670324C181199279 @default.
- W1491670324 hasConceptScore W1491670324C185592680 @default.
- W1491670324 hasConceptScore W1491670324C187882448 @default.
- W1491670324 hasConceptScore W1491670324C20950039 @default.
- W1491670324 hasConceptScore W1491670324C2779123261 @default.
- W1491670324 hasConceptScore W1491670324C54009773 @default.
- W1491670324 hasConceptScore W1491670324C547475151 @default.
- W1491670324 hasConceptScore W1491670324C55493867 @default.
- W1491670324 hasConceptScore W1491670324C86803240 @default.
- W1491670324 hasIssue "46" @default.
- W1491670324 hasLocation W14916703241 @default.
- W1491670324 hasOpenAccess W1491670324 @default.
- W1491670324 hasPrimaryLocation W14916703241 @default.
- W1491670324 hasRelatedWork W2290989990 @default.
- W1491670324 hasRelatedWork W2338137066 @default.
- W1491670324 hasRelatedWork W2352258017 @default.
- W1491670324 hasRelatedWork W2367986948 @default.
- W1491670324 hasRelatedWork W2376774677 @default.
- W1491670324 hasRelatedWork W2384261171 @default.
- W1491670324 hasRelatedWork W2386240997 @default.
- W1491670324 hasRelatedWork W2394346474 @default.
- W1491670324 hasRelatedWork W2410597369 @default.
- W1491670324 hasRelatedWork W2416204398 @default.
- W1491670324 hasVolume "269" @default.
- W1491670324 isParatext "false" @default.
- W1491670324 isRetracted "false" @default.
- W1491670324 magId "1491670324" @default.
- W1491670324 workType "article" @default.