FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1492352549> ?p ?o ?g. }

• W1492352549 endingPage "14044" @default.
• W1492352549 startingPage "14035" @default.
• W1492352549 abstract "Pseudomonas testosteroni protocatechuate 4,5-dioxygenase and Pseudomonas putida catechol 2,3-dioxygenase (metapyrocatechase) catalyze extradiol-type oxygenolytic cleavage of the aromatic ring of their substrates. The essential active site Fe2+ of each enzyme binds nitric oxide (NO) to produce an EPR active complex with an electronic spin of S = 3/2. Hyperfine broadening of the EPR resonances of the nitrosyl complexes by 17O-enriched H2O shows that water is bound directly to the Fe2+ in the native enzymes, but is apparently displaced in substrate complexes. NO is not displaced by either substrates or inhibitors. The EPR spectra of several enzyme-inhibitor-NO complexes are different from those of enzyme-NO or enzyme-substrate-NO complexes and are found to be broadened by 17O-enriched water. The data show that at least 2 and perhaps 3 sites in the Fe ligation can be occupied by exogenous ligands. Furthermore, it is likely that substrates and inhibitors displace water by binding either at or near to the Fe in the nitrosyl complex. Nitric oxide binding is found to be substrate-dependent for each enzyme. Native catechol 2,3-dioxygenase exhibits KD values of 190 microM and 2.0 mM for NO binding in two types of independent sites. Only one type of site is observed in the catechol complex which exhibits a KD for NO of 3.4 microM. One type of NO binding site is observed for both the native and substrate complexed protocatechuate 4,5-dioxygenase with KD values of 360 and 3 microM, respectively. The presence of a specific site in the Fe coordination for NO which is modified in the substrate complex, suggests that O2 binding by the extradiol dioxygenases may also occur at the Fe." @default.
• W1492352549 created "2016-06-24" @default.
• W1492352549 creator A5016562586 @default.
• W1492352549 creator A5019917073 @default.
• W1492352549 creator A5057205939 @default.
• W1492352549 date "1985-11-01" @default.
• W1492352549 modified "2023-10-12" @default.
• W1492352549 title "[17O]Water and nitric oxide binding by protocatechuate 4,5-dioxygenase and catechol 2,3-dioxygenase. Evidence for binding of exogenous ligands to the active site Fe2+ of extradiol dioxygenases." @default.
• W1492352549 cites W112354829 @default.
• W1492352549 cites W1509366823 @default.
• W1492352549 cites W1541946496 @default.
• W1492352549 cites W1561118044 @default.
• W1492352549 cites W1566803604 @default.
• W1492352549 cites W1571230619 @default.
• W1492352549 cites W1574835894 @default.
• W1492352549 cites W1577843043 @default.
• W1492352549 cites W1586027043 @default.
• W1492352549 cites W1617709375 @default.
• W1492352549 cites W1661458410 @default.
• W1492352549 cites W1820654417 @default.
• W1492352549 cites W1978664560 @default.
• W1492352549 cites W1984195074 @default.
• W1492352549 cites W1992669953 @default.
• W1492352549 cites W2005531646 @default.
• W1492352549 cites W2006431480 @default.
• W1492352549 cites W2014966142 @default.
• W1492352549 cites W2017103595 @default.
• W1492352549 cites W2021903117 @default.
• W1492352549 cites W2025381595 @default.
• W1492352549 cites W2026196996 @default.
• W1492352549 cites W2046179506 @default.
• W1492352549 cites W2047343174 @default.
• W1492352549 cites W2063503337 @default.
• W1492352549 cites W2076590965 @default.
• W1492352549 cites W2089242115 @default.
• W1492352549 cites W2185822155 @default.
• W1492352549 cites W2397765685 @default.
• W1492352549 cites W2414868583 @default.
• W1492352549 doi "https://doi.org/10.1016/s0021-9258(17)38681-7" @default.
• W1492352549 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2997190" @default.
• W1492352549 hasPublicationYear "1985" @default.
• W1492352549 type Work @default.
• W1492352549 sameAs 1492352549 @default.
• W1492352549 citedByCount "155" @default.
• W1492352549 countsByYear W14923525492012 @default.
• W1492352549 countsByYear W14923525492013 @default.
• W1492352549 countsByYear W14923525492014 @default.
• W1492352549 countsByYear W14923525492015 @default.
• W1492352549 countsByYear W14923525492016 @default.
• W1492352549 countsByYear W14923525492017 @default.
• W1492352549 countsByYear W14923525492019 @default.
• W1492352549 countsByYear W14923525492020 @default.
• W1492352549 countsByYear W14923525492021 @default.
• W1492352549 countsByYear W14923525492022 @default.
• W1492352549 countsByYear W14923525492023 @default.
• W1492352549 crossrefType "journal-article" @default.
• W1492352549 hasAuthorship W1492352549A5016562586 @default.
• W1492352549 hasAuthorship W1492352549A5019917073 @default.
• W1492352549 hasAuthorship W1492352549A5057205939 @default.
• W1492352549 hasBestOaLocation W14923525491 @default.
• W1492352549 hasConcept C107824862 @default.
• W1492352549 hasConcept C121332964 @default.
• W1492352549 hasConcept C165474305 @default.
• W1492352549 hasConcept C181199279 @default.
• W1492352549 hasConcept C185592680 @default.
• W1492352549 hasConcept C187961010 @default.
• W1492352549 hasConcept C18903297 @default.
• W1492352549 hasConcept C2776834422 @default.
• W1492352549 hasConcept C2777289219 @default.
• W1492352549 hasConcept C2777713698 @default.
• W1492352549 hasConcept C2780543182 @default.
• W1492352549 hasConcept C41183919 @default.
• W1492352549 hasConcept C46141821 @default.
• W1492352549 hasConcept C55493867 @default.
• W1492352549 hasConcept C71240020 @default.
• W1492352549 hasConcept C86803240 @default.
• W1492352549 hasConceptScore W1492352549C107824862 @default.
• W1492352549 hasConceptScore W1492352549C121332964 @default.
• W1492352549 hasConceptScore W1492352549C165474305 @default.
• W1492352549 hasConceptScore W1492352549C181199279 @default.
• W1492352549 hasConceptScore W1492352549C185592680 @default.
• W1492352549 hasConceptScore W1492352549C187961010 @default.
• W1492352549 hasConceptScore W1492352549C18903297 @default.
• W1492352549 hasConceptScore W1492352549C2776834422 @default.
• W1492352549 hasConceptScore W1492352549C2777289219 @default.
• W1492352549 hasConceptScore W1492352549C2777713698 @default.
• W1492352549 hasConceptScore W1492352549C2780543182 @default.
• W1492352549 hasConceptScore W1492352549C41183919 @default.
• W1492352549 hasConceptScore W1492352549C46141821 @default.
• W1492352549 hasConceptScore W1492352549C55493867 @default.
• W1492352549 hasConceptScore W1492352549C71240020 @default.
• W1492352549 hasConceptScore W1492352549C86803240 @default.
• W1492352549 hasIssue "26" @default.
• W1492352549 hasLocation W14923525491 @default.
• W1492352549 hasOpenAccess W1492352549 @default.
• W1492352549 hasPrimaryLocation W14923525491 @default.
• W1492352549 hasRelatedWork W1492352549 @default.
• W1492352549 hasRelatedWork W1971157547 @default.

• next