FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1493089377> ?p ?o ?g. }

• W1493089377 endingPage "8098" @default.
• W1493089377 startingPage "8091" @default.
• W1493089377 abstract "The side chain of residue threonine 65 within the active site of ribulosebisphosphate carboxylase participates in a network of hydrogen bonds and ionic interactions involving the phosphate moiety attached to C-1 of the substrate. This residue was replaced with serine, alanine, and valine in the enzyme from Synechococcus PCC 6301. The mutant enzymes were stable, expressed abundantly by Escherichia coli, and retained the ability to form gel-filterable complexes with the reaction-intermediate analog, 2'-carboxyarabinitol-1,5-bisphosphate. The substitutions reduced the kcat/Km(CO2) (where kcat is the substrate-saturated turnover rate) of the enzyme from 17- to 340-fold with the more radical substitutions causing more severe reductions. The CO2/O2 specificity also deteriorated progressively, the valine replacement causing a 2.3-fold reduction. In concert with these changes, a compound tentatively identified as 1-deoxy-D-glycero-2,3-pentodiulose-5-phosphate, the product of beta elimination of the 2,3-enediol(ate) intermediate of the catalytic reaction, appeared among the reaction products in progressively increasing amounts. In the case of the valine substitution, it comprised 13% of the ribulose bisphosphate consumed. The mutant enzymes also partitioned more of their reaction flux to pentulose bisphosphate isomers of ribulose bisphosphate. By contrast, the diversion of carboxylated product to pyruvate, as a result of beta elimination of the three-carbon aci-carbanion intermediate of the carboxylation reaction, was ameliorated by the replacements, the valine mutant showing a 5-fold improvement in this parameter. These observations focus attention on a geometric conflict which exists between the requirements for stabilization of the 5-carbon enediol(ate) and 3-carbon aci-carbanion intermediates. This conflict must be resolved by a change in the angle of the C-1/bridge oxygen bond during each catalytic cycle. The network of hydrogen bonds involving the side chain of threonine 65 must play a crucial role in facilitating reaction of the enediol(ate) with the gaseous substrate and in shepherding this subsequent movement." @default.
• W1493089377 created "2016-06-24" @default.
• W1493089377 creator A5029871458 @default.
• W1493089377 creator A5035073735 @default.
• W1493089377 creator A5039318995 @default.
• W1493089377 creator A5040306372 @default.
• W1493089377 creator A5062875570 @default.
• W1493089377 date "1994-03-01" @default.
• W1493089377 modified "2023-10-18" @default.
• W1493089377 title "Mutations of an active site threonyl residue promote beta elimination and other side reactions of the enediol intermediate of the ribulosebisphosphate carboxylase reaction." @default.
• W1493089377 cites W122985180 @default.
• W1493089377 cites W1481404114 @default.
• W1493089377 cites W1484420533 @default.
• W1493089377 cites W1492568641 @default.
• W1493089377 cites W1514373528 @default.
• W1493089377 cites W1534304720 @default.
• W1493089377 cites W1567408625 @default.
• W1493089377 cites W1576321072 @default.
• W1493089377 cites W1587613188 @default.
• W1493089377 cites W1589981507 @default.
• W1493089377 cites W1655141131 @default.
• W1493089377 cites W1831600860 @default.
• W1493089377 cites W1966401991 @default.
• W1493089377 cites W1966626008 @default.
• W1493089377 cites W1981894496 @default.
• W1493089377 cites W1985826337 @default.
• W1493089377 cites W1992103258 @default.
• W1493089377 cites W1997094016 @default.
• W1493089377 cites W2006071442 @default.
• W1493089377 cites W2011672515 @default.
• W1493089377 cites W2020119964 @default.
• W1493089377 cites W2023129627 @default.
• W1493089377 cites W2023260542 @default.
• W1493089377 cites W2025296270 @default.
• W1493089377 cites W2026019482 @default.
• W1493089377 cites W2031744738 @default.
• W1493089377 cites W2034148066 @default.
• W1493089377 cites W2039998164 @default.
• W1493089377 cites W2043181907 @default.
• W1493089377 cites W2046696671 @default.
• W1493089377 cites W2053710096 @default.
• W1493089377 cites W2054822429 @default.
• W1493089377 cites W2064034713 @default.
• W1493089377 cites W2070374821 @default.
• W1493089377 cites W2075426147 @default.
• W1493089377 cites W2077492725 @default.
• W1493089377 cites W2089863436 @default.
• W1493089377 cites W2092963159 @default.
• W1493089377 cites W2093617423 @default.
• W1493089377 cites W2128108834 @default.
• W1493089377 cites W2151799162 @default.
• W1493089377 cites W2492469760 @default.
• W1493089377 doi "https://doi.org/10.1016/s0021-9258(17)37164-8" @default.
• W1493089377 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8132534" @default.
• W1493089377 hasPublicationYear "1994" @default.
• W1493089377 type Work @default.
• W1493089377 sameAs 1493089377 @default.
• W1493089377 citedByCount "39" @default.
• W1493089377 countsByYear W14930893772014 @default.
• W1493089377 countsByYear W14930893772016 @default.
• W1493089377 countsByYear W14930893772018 @default.
• W1493089377 countsByYear W14930893772019 @default.
• W1493089377 countsByYear W14930893772022 @default.
• W1493089377 crossrefType "journal-article" @default.
• W1493089377 hasAuthorship W1493089377A5029871458 @default.
• W1493089377 hasAuthorship W1493089377A5035073735 @default.
• W1493089377 hasAuthorship W1493089377A5039318995 @default.
• W1493089377 hasAuthorship W1493089377A5040306372 @default.
• W1493089377 hasAuthorship W1493089377A5062875570 @default.
• W1493089377 hasBestOaLocation W14930893771 @default.
• W1493089377 hasConcept C178790620 @default.
• W1493089377 hasConcept C181199279 @default.
• W1493089377 hasConcept C185592680 @default.
• W1493089377 hasConcept C204921945 @default.
• W1493089377 hasConcept C2781338088 @default.
• W1493089377 hasConcept C41183919 @default.
• W1493089377 hasConcept C521977710 @default.
• W1493089377 hasConcept C55493867 @default.
• W1493089377 hasConcept C71240020 @default.
• W1493089377 hasConceptScore W1493089377C178790620 @default.
• W1493089377 hasConceptScore W1493089377C181199279 @default.
• W1493089377 hasConceptScore W1493089377C185592680 @default.
• W1493089377 hasConceptScore W1493089377C204921945 @default.
• W1493089377 hasConceptScore W1493089377C2781338088 @default.
• W1493089377 hasConceptScore W1493089377C41183919 @default.
• W1493089377 hasConceptScore W1493089377C521977710 @default.
• W1493089377 hasConceptScore W1493089377C55493867 @default.
• W1493089377 hasConceptScore W1493089377C71240020 @default.
• W1493089377 hasIssue "11" @default.
• W1493089377 hasLocation W14930893771 @default.
• W1493089377 hasOpenAccess W1493089377 @default.
• W1493089377 hasPrimaryLocation W14930893771 @default.
• W1493089377 hasRelatedWork W1930059136 @default.
• W1493089377 hasRelatedWork W1959032446 @default.
• W1493089377 hasRelatedWork W2002330103 @default.
• W1493089377 hasRelatedWork W2006946696 @default.
• W1493089377 hasRelatedWork W2018809062 @default.
• W1493089377 hasRelatedWork W2029168180 @default.

• next