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- W1493216518 abstract "Calcium plays prominent roles in regulating a broad range of physiological events in reproduction. The aim of this study was to describe the subcellular distribution of calcium deposits during stages of oogenesis in zebrafish using a combined oxalate–pyroantimonate technique. The oocyte development of zebrafish was categorized into four stages: primary growth, cortical-alveolus, vitellogenic, and maturation, based on morphological criteria. Calcium deposits in the primary growth stage were detected in the cytoplasm, mitochondria, nucleus, and follicular cells. At the cortical-alveolus stage, calcium particles were transported from follicular cells and deposited in the cortical alveoli. In the vitellogenic stage, some cortical alveoli were compacted and transformed from flocculent electron-lucent to electron-dense objects with the progression of the stage. Calcium deposits were transformed from larger to smaller particles, coinciding with compaction of cortical alveoli. In the maturation stage, calcium deposits in all oocyte compartments decreased, with the exception of those in mitochondria. The proportion of area covered by calcium deposits in the mitochondria and cortical alveoli of oocytes at different stages of development was significantly different (p < 0.05). The extent of calcium deposits in the cortical alveoli of mature oocytes was substantially lower than in earlier stages. Basic information about calcium distribution during zebrafish oogenesis may contribute to better understanding of its role in oogenesis." @default.
- W1493216518 created "2016-06-24" @default.
- W1493216518 creator A5017690299 @default.
- W1493216518 creator A5042395335 @default.
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- W1493216518 date "2016-01-01" @default.
- W1493216518 modified "2023-09-23" @default.
- W1493216518 title "Subcellular localization of calcium deposits during zebrafish (Danio rerio) oogenesis" @default.
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- W1493216518 doi "https://doi.org/10.1016/j.micron.2015.09.004" @default.
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- W1493216518 hasPublicationYear "2016" @default.
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