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• W1493290470 abstract "Cytosolic thymidine kinase (EC 2.7.1.21) has been purified 5200-fold to apparent homogeneity from normal human placenta. The purification includes sequential affinity chromatography on blue-Sepharose and a thymidine column. The molecular weight of the enzyme determined by gel filtration and sucrose density ultracentrifugation is 92,000. The subunit molecular weight is 44,000, suggesting that the enzyme is a dimer in its native state. With isoelectric focusing, placental thymidine kinase demonstrated a single form with an isoelectric point of 9.1. The final purified enzyme preparation exhibits no immunological cross-reactivity with human mitochondrial thymidine kinase." @default.
• W1493290470 created "2016-06-24" @default.
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• W1493290470 date "1983-06-01" @default.
• W1493290470 modified "2023-09-30" @default.
• W1493290470 title "Human thymidine kinase. Purification and properties of the cytosolic enzyme of placenta." @default.
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• W1493290470 doi "https://doi.org/10.1016/s0021-9258(18)32324-x" @default.
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