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• W1493365190 abstract "Background and purpose: Our previous study showed that urocortin (Ucn1) exacerbates the hypercoagulable state and vasculitis in a rat model of sodium laurate‐induced thromboangiitis obliterans. Furthermore, the inflammatory molecules COX‐2 and ICAM‐1 may participate in this effect. In the present study, the effects of Ucn1 on COX‐2 and ICAM‐1 expression in lipopolysaccharide (LPS)‐induced rat aortic endothelial cells (RAECs) were investigated and the mechanisms involved explored. Experimental approach: RAECs were isolated from adult male Wistar rats, and identified at the first passage. Experiments were performed on cells, from primary culture, at passages 5–8. The expression of COX‐2 and ICAM‐1 at both mRNA and protein levels was determined by semi‐quantitative RT‐PCR and Western blot analysis. Levels of PGE 2 and soluble ICAM‐1 (sICAM‐1) in culture medium were measured by enzyme‐linked immunosorbent assay. Furthermore, the phosphorylation status of p38MAPK, ERK1/2, JNK, Akt and NF‐κB was analysed by Western blot; nuclear translocation of NF‐κB was observed by immunofluorescence. Key results: Ucn1 augmented LPS‐induced expression of COX‐2 and ICAM‐1 in RAECs in a time‐ and concentration‐dependent manner. Ucn1 increased PGE 2 and sICAM‐1 levels. These effects were abolished by the CRF 2 receptor antagonist, antisauvagine‐30, but not by the CRF 1 receptor antagonist, NBI‐27914. Moreover, Ucn2 activated p38MAPK and augmented NF‐κB nuclear translocation and phosphorylation, whereas ERK1/2, JNK and Akt pathways were not involved in this process. Conclusions and implications: These findings suggest that Ucn1 exerts pro‐inflammatory effects by augmenting LPS‐induced expression of COX‐2 and ICAM‐1 in RAECs via CRF 2 receptors and the activation of p38MAPK and NF‐κB." @default.
• W1493365190 created "2016-06-24" @default.
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• W1493365190 date "2009-10-01" @default.
• W1493365190 modified "2023-10-17" @default.
• W1493365190 title "Urocortin induced expression of COX-2 and ICAM-1 via corticotrophin-releasing factor type 2 receptor in rat aortic endothelial cells" @default.
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• W1493365190 doi "https://doi.org/10.1111/j.1476-5381.2009.00346.x" @default.
• W1493365190 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2765601" @default.
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