Matches in SemOpenAlex for { <https://semopenalex.org/work/W1493442995> ?p ?o ?g. }
Showing items 1 to 94 of
94
with 100 items per page.
- W1493442995 endingPage "528" @default.
- W1493442995 startingPage "519" @default.
- W1493442995 abstract "The interactions of simple metal complexes with DNA have been widely studied. The interaction specificities of these molecules range from simple outside binding, such as observed with [Co(NH3)6]3+, to covalent binding as observed with Pt(NH3)2Cl2, to reactions involving DNA oxidative cleavage through Fenton or related chemistries. One important feature of such interactions, especially those leading to covalent binding, is the sequence specificity of the reaction. This chapter presents a facile and fast method that can be employed to assess the sequence specificity of a ligand's reaction with DNA. Although the reagent used here covalently binds to DNA, this method should also work for reversible interactions as well, as long as the ligand has a high affinity for the DNA. To assess the sequence specificity, if any, of the reaction of [Co(NH3)5(OH2)]3+ with DNA, a series of four different model DNA oligomers was synthesized and treated with the cobalt complex according to established protocols. Each oligomer is self-complementary and possesses a unique restriction endonuclease site containing one or two G bases. A thermodynamic approach was used to assess the sequence specificity of the interaction. To summarize the results of these studies, each oligomer was incubated with [Co(NH3)5(OH2)]3+ and then exhaustively dialyzed to remove nonspecifically bound cobalt complex." @default.
- W1493442995 created "2016-06-24" @default.
- W1493442995 creator A5018820370 @default.
- W1493442995 creator A5040129249 @default.
- W1493442995 date "2001-01-01" @default.
- W1493442995 modified "2023-09-25" @default.
- W1493442995 title "Locating cobalt-binding sites on dna using restriction endonucleases" @default.
- W1493442995 cites W1603675492 @default.
- W1493442995 cites W1983946766 @default.
- W1493442995 cites W1988619957 @default.
- W1493442995 cites W2002018714 @default.
- W1493442995 cites W2015553561 @default.
- W1493442995 cites W2020610229 @default.
- W1493442995 cites W2028337326 @default.
- W1493442995 cites W2059424224 @default.
- W1493442995 cites W2059678562 @default.
- W1493442995 cites W2069983479 @default.
- W1493442995 cites W2437067295 @default.
- W1493442995 cites W2952816712 @default.
- W1493442995 doi "https://doi.org/10.1016/s0076-6879(01)40440-x" @default.
- W1493442995 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11494868" @default.
- W1493442995 hasPublicationYear "2001" @default.
- W1493442995 type Work @default.
- W1493442995 sameAs 1493442995 @default.
- W1493442995 citedByCount "4" @default.
- W1493442995 countsByYear W14934429952012 @default.
- W1493442995 countsByYear W14934429952013 @default.
- W1493442995 crossrefType "book-chapter" @default.
- W1493442995 hasAuthorship W1493442995A5018820370 @default.
- W1493442995 hasAuthorship W1493442995A5040129249 @default.
- W1493442995 hasConcept C100594029 @default.
- W1493442995 hasConcept C107824862 @default.
- W1493442995 hasConcept C116569031 @default.
- W1493442995 hasConcept C128319531 @default.
- W1493442995 hasConcept C151730666 @default.
- W1493442995 hasConcept C170493617 @default.
- W1493442995 hasConcept C175156509 @default.
- W1493442995 hasConcept C178790620 @default.
- W1493442995 hasConcept C180577832 @default.
- W1493442995 hasConcept C185592680 @default.
- W1493442995 hasConcept C188027245 @default.
- W1493442995 hasConcept C21951064 @default.
- W1493442995 hasConcept C2777028655 @default.
- W1493442995 hasConcept C2778112365 @default.
- W1493442995 hasConcept C2780642029 @default.
- W1493442995 hasConcept C40875361 @default.
- W1493442995 hasConcept C43369102 @default.
- W1493442995 hasConcept C515602321 @default.
- W1493442995 hasConcept C552990157 @default.
- W1493442995 hasConcept C55493867 @default.
- W1493442995 hasConcept C71240020 @default.
- W1493442995 hasConcept C86803240 @default.
- W1493442995 hasConceptScore W1493442995C100594029 @default.
- W1493442995 hasConceptScore W1493442995C107824862 @default.
- W1493442995 hasConceptScore W1493442995C116569031 @default.
- W1493442995 hasConceptScore W1493442995C128319531 @default.
- W1493442995 hasConceptScore W1493442995C151730666 @default.
- W1493442995 hasConceptScore W1493442995C170493617 @default.
- W1493442995 hasConceptScore W1493442995C175156509 @default.
- W1493442995 hasConceptScore W1493442995C178790620 @default.
- W1493442995 hasConceptScore W1493442995C180577832 @default.
- W1493442995 hasConceptScore W1493442995C185592680 @default.
- W1493442995 hasConceptScore W1493442995C188027245 @default.
- W1493442995 hasConceptScore W1493442995C21951064 @default.
- W1493442995 hasConceptScore W1493442995C2777028655 @default.
- W1493442995 hasConceptScore W1493442995C2778112365 @default.
- W1493442995 hasConceptScore W1493442995C2780642029 @default.
- W1493442995 hasConceptScore W1493442995C40875361 @default.
- W1493442995 hasConceptScore W1493442995C43369102 @default.
- W1493442995 hasConceptScore W1493442995C515602321 @default.
- W1493442995 hasConceptScore W1493442995C552990157 @default.
- W1493442995 hasConceptScore W1493442995C55493867 @default.
- W1493442995 hasConceptScore W1493442995C71240020 @default.
- W1493442995 hasConceptScore W1493442995C86803240 @default.
- W1493442995 hasLocation W14934429951 @default.
- W1493442995 hasLocation W14934429952 @default.
- W1493442995 hasOpenAccess W1493442995 @default.
- W1493442995 hasPrimaryLocation W14934429951 @default.
- W1493442995 hasRelatedWork W1493442995 @default.
- W1493442995 hasRelatedWork W1967663354 @default.
- W1493442995 hasRelatedWork W1989443100 @default.
- W1493442995 hasRelatedWork W2038460592 @default.
- W1493442995 hasRelatedWork W2058046308 @default.
- W1493442995 hasRelatedWork W2088308136 @default.
- W1493442995 hasRelatedWork W2150202659 @default.
- W1493442995 hasRelatedWork W2395355812 @default.
- W1493442995 hasRelatedWork W2739147944 @default.
- W1493442995 hasRelatedWork W4292253172 @default.
- W1493442995 isParatext "false" @default.
- W1493442995 isRetracted "false" @default.
- W1493442995 magId "1493442995" @default.
- W1493442995 workType "book-chapter" @default.