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• W1494106285 endingPage "3414" @default.
• W1494106285 startingPage "3407" @default.
• W1494106285 abstract "To study correlations between cellular transformation and the biochemical properties of polyomavirus middle T antigen, middle T cDNAs have been derived from the polyomavirus mutants dl1015, dl23, and NG59b and have been introduced into rodent fibroblast cell lines by using a retrovirus vector. It was found that all three mutants are completely defective in inducing growth in soft agar but possess a range of activities in assays of focus formation on cell monolayers. Furthermore, when assays of middle T antigen-associated kinase activities were performed in vitro, a correlation between the level of associated phosphatidylinositol kinase activity and the ability of mutant middle T antigens to induce focus formation was observed. However, the association of this activity with middle T antigen does not appear to be sufficient to bring about full transformation, since the middle T antigen derived from dl1015 is completely defective for soft-agar growth but is associated with a level of phosphatidylinositol kinase activity which is comparable to that of the wild type. Therefore, some other unidentified middle T antigen function may also be required for full transformation." @default.
• W1494106285 created "2016-06-24" @default.
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• W1494106285 date "1988-09-01" @default.
• W1494106285 modified "2023-10-16" @default.
• W1494106285 title "Recombinant retroviruses that transduce middle T antigen cDNAs derived from polyomavirus mutants: separation of focus formation and soft-agar growth in transformation assays and correlations with kinase activities in vitro" @default.
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• W1494106285 doi "https://doi.org/10.1128/jvi.62.9.3407-3414.1988" @default.
• W1494106285 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/253464" @default.
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• W1494106285 hasPublicationYear "1988" @default.
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