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- W1495719083 abstract "Abstract Cellular morphology is inherently three‐dimensional. However, most histological techniques for tissue analysis focus on extracting information from two‐dimensional slices of fixed samples or dissociated cells. These techniques result in a significant loss of the three‐dimensional information of the tissue, including true cell volume, orientation, and whole cell shape. This unit discusses various options for three‐dimensional imaging, provides a protocol for performing post‐processing reconstruction based on serial slicing, and discusses the current advantages and limitations of the three‐dimensional approach to quantitative tissue analysis. The focus of this protocol is on cardiac tissue, but the techniques can be applied to any solid tissue." @default.
- W1495719083 created "2016-06-24" @default.
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- W1495719083 date "2006-04-01" @default.
- W1495719083 modified "2023-10-12" @default.
- W1495719083 title "Three‐Dimensional Reconstruction of Tissues" @default.
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- W1495719083 doi "https://doi.org/10.1002/0471142727.mb1416s74" @default.
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