FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1495897262> ?p ?o ?g. }

• W1495897262 endingPage "19162" @default.
• W1495897262 startingPage "19155" @default.
• W1495897262 abstract "The region including the conserved Ser65-Asp66 dipeptide in the tetracycline/H+ antiporter (TET) encoded by transposon Tn10 is thought to play a gating role (Yamaguchi, A., Ono, N., Akasaka, T., Noumi, T., and Sawai, T. (1990) J. Biol. Chem. 265, 15525-15530). The dipeptide is in putative interhelix loop2-3, which also includes the conserved sequence motif, GXXXXRXGRR, found in all TET proteins and sugar/H+ symporters. Through the combination of localized random and site-directed mutagenesis, each residue in loop2-3 was replaced. Among 10 residues in putative loop2-3, the important residues, of which substitution resulted in significant reduction or complete loss of the transport activity, were Gly62, Asp66, Gly69, and Arg70. The defect in the transport activity of the Gly62 and Gly69 substitution mutants corresponded to the steric hindrance by the substituents as to the putative beta-turn structure of the peptide backbone containing these glycines. Of 3 conserved Arg residues, the replacement of only Arg70 caused complete loss of the activity except for replacement with Lys, indicating the importance of a positive charge at this position, which is similar to the essentiality of a negative charge at Asp66. A charge-neutralizing intra-loop salt bridge between Asp66 and Arg70 was not likely because the double mutant in which Asp66 and Arg70 were replaced with asparagine and leucine, respectively, showed no transport activity. A triple mutant with only one positive charge at Arg70 in this loop showed about half the wild-type activity, indicating that the polycationic nature of the loop was not critical for the activity. Cys mutants as to the unessential residues in the loop were modifiable with N-ethylmaleimide, except for the Met64----Cys and Arg71----Cys mutants; however, the modification of only the Ser65----Cys mutant caused significant inhibition of the transport activity, indicating that position 65 is a unique position in the structure of loop2-3." @default.
• W1495897262 created "2016-06-24" @default.
• W1495897262 creator A5012013197 @default.
• W1495897262 creator A5052384578 @default.
• W1495897262 creator A5086235952 @default.
• W1495897262 date "1992-09-01" @default.
• W1495897262 modified "2023-09-29" @default.
• W1495897262 title "Metal-tetracycline/H+ antiporter of Escherichia coli encoded by transposon Tn10. The role of a conserved sequence motif, GXXXXRXGRR, in a putative cytoplasmic loop between helices 2 and 3." @default.
• W1495897262 cites W1526170391 @default.
• W1495897262 cites W1532704140 @default.
• W1495897262 cites W1543135651 @default.
• W1495897262 cites W1555021163 @default.
• W1495897262 cites W1561081856 @default.
• W1495897262 cites W1566208554 @default.
• W1495897262 cites W1570747676 @default.
• W1495897262 cites W1602240975 @default.
• W1495897262 cites W1991758707 @default.
• W1495897262 cites W1996066959 @default.
• W1495897262 cites W1997399405 @default.
• W1495897262 cites W2000284372 @default.
• W1495897262 cites W2003930046 @default.
• W1495897262 cites W2024830628 @default.
• W1495897262 cites W2049695588 @default.
• W1495897262 cites W2059169742 @default.
• W1495897262 cites W2071495824 @default.
• W1495897262 cites W2073882280 @default.
• W1495897262 cites W2077703362 @default.
• W1495897262 cites W2091789402 @default.
• W1495897262 cites W2117476471 @default.
• W1495897262 cites W2301861019 @default.
• W1495897262 doi "https://doi.org/10.1016/s0021-9258(18)41755-3" @default.
• W1495897262 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1326546" @default.
• W1495897262 hasPublicationYear "1992" @default.
• W1495897262 type Work @default.
• W1495897262 sameAs 1495897262 @default.
• W1495897262 citedByCount "93" @default.
• W1495897262 countsByYear W14958972622013 @default.
• W1495897262 countsByYear W14958972622014 @default.
• W1495897262 countsByYear W14958972622015 @default.
• W1495897262 countsByYear W14958972622016 @default.
• W1495897262 countsByYear W14958972622017 @default.
• W1495897262 countsByYear W14958972622018 @default.
• W1495897262 countsByYear W14958972622021 @default.
• W1495897262 countsByYear W14958972622022 @default.
• W1495897262 crossrefType "journal-article" @default.
• W1495897262 hasAuthorship W1495897262A5012013197 @default.
• W1495897262 hasAuthorship W1495897262A5052384578 @default.
• W1495897262 hasAuthorship W1495897262A5086235952 @default.
• W1495897262 hasBestOaLocation W14958972621 @default.
• W1495897262 hasConcept C101425835 @default.
• W1495897262 hasConcept C104317684 @default.
• W1495897262 hasConcept C117745874 @default.
• W1495897262 hasConcept C143065580 @default.
• W1495897262 hasConcept C157887575 @default.
• W1495897262 hasConcept C167625842 @default.
• W1495897262 hasConcept C185592680 @default.
• W1495897262 hasConcept C199216141 @default.
• W1495897262 hasConcept C2778910516 @default.
• W1495897262 hasConcept C2780923616 @default.
• W1495897262 hasConcept C41625074 @default.
• W1495897262 hasConcept C4918238 @default.
• W1495897262 hasConcept C501593827 @default.
• W1495897262 hasConcept C54355233 @default.
• W1495897262 hasConcept C547475151 @default.
• W1495897262 hasConcept C55493867 @default.
• W1495897262 hasConcept C57181701 @default.
• W1495897262 hasConcept C86803240 @default.
• W1495897262 hasConceptScore W1495897262C101425835 @default.
• W1495897262 hasConceptScore W1495897262C104317684 @default.
• W1495897262 hasConceptScore W1495897262C117745874 @default.
• W1495897262 hasConceptScore W1495897262C143065580 @default.
• W1495897262 hasConceptScore W1495897262C157887575 @default.
• W1495897262 hasConceptScore W1495897262C167625842 @default.
• W1495897262 hasConceptScore W1495897262C185592680 @default.
• W1495897262 hasConceptScore W1495897262C199216141 @default.
• W1495897262 hasConceptScore W1495897262C2778910516 @default.
• W1495897262 hasConceptScore W1495897262C2780923616 @default.
• W1495897262 hasConceptScore W1495897262C41625074 @default.
• W1495897262 hasConceptScore W1495897262C4918238 @default.
• W1495897262 hasConceptScore W1495897262C501593827 @default.
• W1495897262 hasConceptScore W1495897262C54355233 @default.
• W1495897262 hasConceptScore W1495897262C547475151 @default.
• W1495897262 hasConceptScore W1495897262C55493867 @default.
• W1495897262 hasConceptScore W1495897262C57181701 @default.
• W1495897262 hasConceptScore W1495897262C86803240 @default.
• W1495897262 hasIssue "27" @default.
• W1495897262 hasLocation W14958972621 @default.
• W1495897262 hasOpenAccess W1495897262 @default.
• W1495897262 hasPrimaryLocation W14958972621 @default.
• W1495897262 hasRelatedWork W1495897262 @default.
• W1495897262 hasRelatedWork W1561081856 @default.
• W1495897262 hasRelatedWork W1599169047 @default.
• W1495897262 hasRelatedWork W1866871634 @default.
• W1495897262 hasRelatedWork W1942326742 @default.
• W1495897262 hasRelatedWork W1977884124 @default.
• W1495897262 hasRelatedWork W2054329803 @default.
• W1495897262 hasRelatedWork W2062668071 @default.
• W1495897262 hasRelatedWork W2171277850 @default.

• next