FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1496007797> ?p ?o ?g. }

• W1496007797 endingPage "263" @default.
• W1496007797 startingPage "253" @default.
• W1496007797 abstract "Rotating-wall vessels are beneficial to tissue engineering in that the reconstituted tissue formed in these low-shear bioreactors undergoes extensive three-dimensional growth and differentiation. In the present study, bovine corneal endothelial (BCE) cells were grown in a high-aspect rotating-wall vessel (HARV) attached to collagen-coated Cytodex-3 beads as a representative monolayer culture to investigate factors during HARV cultivation which affect three-dimensional growth and protein expression. A collagen type I substratum in T-flask control cultures increased cell density of BCE cells at confluence by 40% and altered the expression of select proteins (43, 50 and 210 kDa). The low-shear environment in the HARV facilitated cell bridging between microcarrier beads to form aggregates containing upwards of 23 beads each, but it did not promote multilayer growth. A kinetic model of microcarrier aggregation was developed which indicates that the rate of aggregation between a single bead and an aggregate was nearly 10 times faster than between two aggregate and 60 times faster than between two single beads. These differences reflect changes in collision frequency and cell bridge formation. HARV cultivation altered the expression of cellular proteins (43 and 70 kDa) and matrix proteins (50, 73, 89 and 210 kDa) relative to controls perhaps due to hypoxia, fluid flow or distortion of cell shape. In addition to the insight that this work has provided into rotating-wall vessels, it could be useful in modeling aggregation in other cell systems, propagating human corneal endothelial cells for eye surgery and examining the response of endothelial cells to reduced shear." @default.
• W1496007797 created "2016-06-24" @default.
• W1496007797 creator A5023072035 @default.
• W1496007797 creator A5032881793 @default.
• W1496007797 creator A5042172399 @default.
• W1496007797 creator A5056894495 @default.
• W1496007797 creator A5056945472 @default.
• W1496007797 creator A5069188329 @default.
• W1496007797 date "2000-01-01" @default.
• W1496007797 modified "2023-10-18" @default.
• W1496007797 cites W1488597819 @default.
• W1496007797 cites W1587900371 @default.
• W1496007797 cites W1963720608 @default.
• W1496007797 cites W1981258806 @default.
• W1496007797 cites W1999487669 @default.
• W1496007797 cites W2007580610 @default.
• W1496007797 cites W2016138335 @default.
• W1496007797 cites W2021534360 @default.
• W1496007797 cites W2022724873 @default.
• W1496007797 cites W2028518884 @default.
• W1496007797 cites W2028721552 @default.
• W1496007797 cites W2028857913 @default.
• W1496007797 cites W2029397320 @default.
• W1496007797 cites W2036703730 @default.
• W1496007797 cites W2037781052 @default.
• W1496007797 cites W2038594750 @default.
• W1496007797 cites W2040703945 @default.
• W1496007797 cites W2061381050 @default.
• W1496007797 cites W2066994762 @default.
• W1496007797 cites W2068253453 @default.
• W1496007797 cites W2071557505 @default.
• W1496007797 cites W2077505292 @default.
• W1496007797 cites W2088765023 @default.
• W1496007797 cites W2090587732 @default.
• W1496007797 cites W2094471657 @default.
• W1496007797 cites W2095725874 @default.
• W1496007797 cites W2100837269 @default.
• W1496007797 cites W2111506239 @default.
• W1496007797 cites W2112457977 @default.
• W1496007797 cites W2113779295 @default.
• W1496007797 cites W2137948742 @default.
• W1496007797 cites W2139521806 @default.
• W1496007797 cites W2164494705 @default.
• W1496007797 cites W2164532298 @default.
• W1496007797 cites W2400880132 @default.
• W1496007797 cites W2431352641 @default.
• W1496007797 cites W567826855 @default.
• W1496007797 cites W1978703392 @default.
• W1496007797 doi "https://doi.org/10.1023/a:1008117410827" @default.
• W1496007797 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3449895" @default.
• W1496007797 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19002986" @default.
• W1496007797 hasPublicationYear "2000" @default.
• W1496007797 type Work @default.
• W1496007797 sameAs 1496007797 @default.
• W1496007797 citedByCount "14" @default.
• W1496007797 countsByYear W14960077972013 @default.
• W1496007797 countsByYear W14960077972014 @default.
• W1496007797 countsByYear W14960077972016 @default.
• W1496007797 countsByYear W14960077972017 @default.
• W1496007797 countsByYear W14960077972022 @default.
• W1496007797 crossrefType "journal-article" @default.
• W1496007797 hasAuthorship W1496007797A5023072035 @default.
• W1496007797 hasAuthorship W1496007797A5032881793 @default.
• W1496007797 hasAuthorship W1496007797A5042172399 @default.
• W1496007797 hasAuthorship W1496007797A5056894495 @default.
• W1496007797 hasAuthorship W1496007797A5056945472 @default.
• W1496007797 hasAuthorship W1496007797A5069188329 @default.
• W1496007797 hasBestOaLocation W14960077972 @default.
• W1496007797 hasConcept C12554922 @default.
• W1496007797 hasConcept C1491633281 @default.
• W1496007797 hasConcept C168170006 @default.
• W1496007797 hasConcept C176999471 @default.
• W1496007797 hasConcept C178790620 @default.
• W1496007797 hasConcept C185592680 @default.
• W1496007797 hasConcept C54355233 @default.
• W1496007797 hasConcept C55493867 @default.
• W1496007797 hasConcept C62112901 @default.
• W1496007797 hasConcept C81885089 @default.
• W1496007797 hasConcept C86803240 @default.
• W1496007797 hasConcept C95444343 @default.
• W1496007797 hasConceptScore W1496007797C12554922 @default.
• W1496007797 hasConceptScore W1496007797C1491633281 @default.
• W1496007797 hasConceptScore W1496007797C168170006 @default.
• W1496007797 hasConceptScore W1496007797C176999471 @default.
• W1496007797 hasConceptScore W1496007797C178790620 @default.
• W1496007797 hasConceptScore W1496007797C185592680 @default.
• W1496007797 hasConceptScore W1496007797C54355233 @default.
• W1496007797 hasConceptScore W1496007797C55493867 @default.
• W1496007797 hasConceptScore W1496007797C62112901 @default.
• W1496007797 hasConceptScore W1496007797C81885089 @default.
• W1496007797 hasConceptScore W1496007797C86803240 @default.
• W1496007797 hasConceptScore W1496007797C95444343 @default.
• W1496007797 hasIssue "3" @default.
• W1496007797 hasLocation W14960077971 @default.
• W1496007797 hasLocation W14960077972 @default.
• W1496007797 hasLocation W14960077973 @default.
• W1496007797 hasLocation W14960077974 @default.
• W1496007797 hasOpenAccess W1496007797 @default.

• next