Matches in SemOpenAlex for { <https://semopenalex.org/work/W1496252103> ?p ?o ?g. }
Showing items 1 to 96 of
96
with 100 items per page.
- W1496252103 endingPage "5" @default.
- W1496252103 startingPage "2141" @default.
- W1496252103 abstract "In meningeal carcinomatosis, retroviral vector-producer cells can be introduced into the thecal sac and circulate in the cerebrospinal fluid to reach malignant tumor cells in the leptomeninges, release vector particles, and selectively infect and transfer a gene of interest to these cells. Gene transfer experiments with the lacZ gene and in vitro retroviral titer measurements showed that retroviral vectors can survive in the cerebrospinal fluid, retain their infectivity, and successfully transduce tumor cells. To examine the potential of intrathecal gene therapy, we evaluated the antitumor efficacy of in situ transduction with the herpes simplex-thymidine kinase gene followed by ganciclovir therapy in a rat model of leptomeningeal neoplasia. Fischer rats were inoculated via a subarachnoid catheter implanted at the upper thoracic level, and thymidine kinase vector-producer cells were injected into the subarachnoid space the day of tumor inoculation. Seven days later, rats received ganciclovir for 14 days by daily i.p. injections (30 mg/kg/ml) or intrathecal injections (25 micrograms/kg or 600 micrograms/kg) for 14 days. To evaluate possible enhancement of tumor eradication by the ability of helper virus to package the vector in the cells and further extend gene transfer, additional rats received thymidine kinase vector-producer cells that had been previously coinfected with a replication-competent retrovirus (4070A). In all groups, control rats received i.p. or intrathecal saline injections. Ganciclovir administration i.p. resulted in significant prolongation of survival in rats given injections of thymidine kinase vector-producer cells. Injection of producer cells coinfected with the 4070A retrovirus did not improve antitumor efficacy. Intrathecal administration of ganciclovir (low and high doses) did not extend survival; histological examination of the spinal cords showed elimination of the infiltrative tumor in the leptomeninges, but residual tumor mass was present at the inoculation site, consistent with limited penetration of topical ganciclovir into the tumor. These results support the potential application of gene therapy using the thymidine kinase/ganciclovir approach for treatment of meningeal carcinomatosis." @default.
- W1496252103 created "2016-06-24" @default.
- W1496252103 creator A5007865413 @default.
- W1496252103 creator A5016461712 @default.
- W1496252103 creator A5039528625 @default.
- W1496252103 creator A5064348664 @default.
- W1496252103 creator A5069618055 @default.
- W1496252103 creator A5072235800 @default.
- W1496252103 creator A5084929123 @default.
- W1496252103 creator A5091529402 @default.
- W1496252103 date "1994-04-15" @default.
- W1496252103 modified "2023-09-27" @default.
- W1496252103 title "Intrathecal gene therapy for malignant leptomeningeal neoplasia." @default.
- W1496252103 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8174120" @default.
- W1496252103 hasPublicationYear "1994" @default.
- W1496252103 type Work @default.
- W1496252103 sameAs 1496252103 @default.
- W1496252103 citedByCount "15" @default.
- W1496252103 crossrefType "journal-article" @default.
- W1496252103 hasAuthorship W1496252103A5007865413 @default.
- W1496252103 hasAuthorship W1496252103A5016461712 @default.
- W1496252103 hasAuthorship W1496252103A5039528625 @default.
- W1496252103 hasAuthorship W1496252103A5064348664 @default.
- W1496252103 hasAuthorship W1496252103A5069618055 @default.
- W1496252103 hasAuthorship W1496252103A5072235800 @default.
- W1496252103 hasAuthorship W1496252103A5084929123 @default.
- W1496252103 hasAuthorship W1496252103A5091529402 @default.
- W1496252103 hasConcept C104317684 @default.
- W1496252103 hasConcept C111599444 @default.
- W1496252103 hasConcept C142724271 @default.
- W1496252103 hasConcept C15152581 @default.
- W1496252103 hasConcept C159047783 @default.
- W1496252103 hasConcept C2522874641 @default.
- W1496252103 hasConcept C2776147627 @default.
- W1496252103 hasConcept C2777965375 @default.
- W1496252103 hasConcept C2778277180 @default.
- W1496252103 hasConcept C2778431562 @default.
- W1496252103 hasConcept C2779651940 @default.
- W1496252103 hasConcept C2779820661 @default.
- W1496252103 hasConcept C2780813603 @default.
- W1496252103 hasConcept C2781196997 @default.
- W1496252103 hasConcept C32470452 @default.
- W1496252103 hasConcept C34981463 @default.
- W1496252103 hasConcept C40767141 @default.
- W1496252103 hasConcept C54009773 @default.
- W1496252103 hasConcept C54355233 @default.
- W1496252103 hasConcept C55493867 @default.
- W1496252103 hasConcept C71924100 @default.
- W1496252103 hasConcept C81885089 @default.
- W1496252103 hasConcept C86803240 @default.
- W1496252103 hasConcept C92087593 @default.
- W1496252103 hasConceptScore W1496252103C104317684 @default.
- W1496252103 hasConceptScore W1496252103C111599444 @default.
- W1496252103 hasConceptScore W1496252103C142724271 @default.
- W1496252103 hasConceptScore W1496252103C15152581 @default.
- W1496252103 hasConceptScore W1496252103C159047783 @default.
- W1496252103 hasConceptScore W1496252103C2522874641 @default.
- W1496252103 hasConceptScore W1496252103C2776147627 @default.
- W1496252103 hasConceptScore W1496252103C2777965375 @default.
- W1496252103 hasConceptScore W1496252103C2778277180 @default.
- W1496252103 hasConceptScore W1496252103C2778431562 @default.
- W1496252103 hasConceptScore W1496252103C2779651940 @default.
- W1496252103 hasConceptScore W1496252103C2779820661 @default.
- W1496252103 hasConceptScore W1496252103C2780813603 @default.
- W1496252103 hasConceptScore W1496252103C2781196997 @default.
- W1496252103 hasConceptScore W1496252103C32470452 @default.
- W1496252103 hasConceptScore W1496252103C34981463 @default.
- W1496252103 hasConceptScore W1496252103C40767141 @default.
- W1496252103 hasConceptScore W1496252103C54009773 @default.
- W1496252103 hasConceptScore W1496252103C54355233 @default.
- W1496252103 hasConceptScore W1496252103C55493867 @default.
- W1496252103 hasConceptScore W1496252103C71924100 @default.
- W1496252103 hasConceptScore W1496252103C81885089 @default.
- W1496252103 hasConceptScore W1496252103C86803240 @default.
- W1496252103 hasConceptScore W1496252103C92087593 @default.
- W1496252103 hasIssue "8" @default.
- W1496252103 hasLocation W14962521031 @default.
- W1496252103 hasOpenAccess W1496252103 @default.
- W1496252103 hasPrimaryLocation W14962521031 @default.
- W1496252103 hasRelatedWork W1496252103 @default.
- W1496252103 hasRelatedWork W1978579854 @default.
- W1496252103 hasRelatedWork W1992947808 @default.
- W1496252103 hasRelatedWork W1994397468 @default.
- W1496252103 hasRelatedWork W2041186543 @default.
- W1496252103 hasRelatedWork W2073876222 @default.
- W1496252103 hasRelatedWork W2166007074 @default.
- W1496252103 hasRelatedWork W2319106492 @default.
- W1496252103 hasRelatedWork W2405007393 @default.
- W1496252103 hasRelatedWork W2405885599 @default.
- W1496252103 hasVolume "54" @default.
- W1496252103 isParatext "false" @default.
- W1496252103 isRetracted "false" @default.
- W1496252103 magId "1496252103" @default.
- W1496252103 workType "article" @default.