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- W1497258452 abstract "Alkaline grass ( Puccinellia tenuiflora ) can serve as a model of salt tolerance in monocotyledon crops. However, little is known about its mechanism of aluminum stress. In this work, we screened PutSTE24 gene under aluminum stress to predict its subcellular localization of proteins by using bioinformatics analysis software, and the result showed that this gene was in the endoplasmic reticulum, which was the same with expressing PutSTE24 gene in yeast INVsI. Under aluminum and saline-alkali stress conditions, the relative mRNA expression levels of the PutSTE24 of alkaline grass were measured by real-time quantitative PCR, and the results demonstrated that its expression was induced up regulation. We also constructed a plant expression vector of PutSTE24 gene, and then transferred into Arabidopsis. For further determine the correlation between PutSTE24 gene and aluminum stress, we choose transgenic seeds for resistance experiment, which provided a wealth of theoretical and experimental basis for further research on interaction of related genes and regulatory mechanism of aluminum stress." @default.
- W1497258452 created "2016-06-24" @default.
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- W1497258452 date "2014-01-01" @default.
- W1497258452 modified "2023-09-27" @default.
- W1497258452 title "Expression Analysis of <i>Puccinellia tenuifolra</i> Gene <i>PutSTE24</i> and Response to Aluminium Stresses" @default.
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- W1497258452 doi "https://doi.org/10.5376/msb.2014.05.0002" @default.
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