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- W1497391707 abstract "Two kinds of specific chicken egg yolk immunoglobulins (IgYs), IgY-WSSV and IgY-VP28, were, respectively, raised against the 2 mM binary ethylenimine (BEI)-inactivated white spot syndrome virus (WSSV) and a principal envelope protein VP28. The activity of purified specific IgYs was stable under the conditions of 20–70 °C, pH 3.0–10.0 and 0–700 g L−1 sucrose solution. In the neutralization assay, these high-affinity IgY antibodies can specifically bind with the virus particles to protect shrimp (Fenneropenaeus chinensis) against WSSV infection. After oral delivery for 20 days, the IgY-WSSV exerted a higher protection effect (RPS: 71.5%) than IgY-VP28 (RPS: 63.7%). Moreover, an increase in RPS (79.2%) was found on addition of IgY-WSSV:VP28 (0.1% IgY-VP28 plus 0.2% IgY-WSSV). This may indicate that neutralization of WSSV refers to the multiple-hit model. By time-course study of the levels of the specific IgYs in vivo, the data showed that the titre was enhanced to a relatively high level (P/N=8.35±0.45) at 3 days post administration, declined slightly (P/N=7.13±1.01) at 7 days post administration and then remained stable for further investigation. The stable antibody level potentially contributes towards blocking a large number of WSSV particles from entering and infecting on the major tissues at the early and late stages after challenge in shrimp." @default.
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- W1497391707 date "2010-11-01" @default.
- W1497391707 modified "2023-09-23" @default.
- W1497391707 title "Protection of Fenneropenaeus chinensis (Osbeck, 1765) against the white spot syndrome virus using specific chicken egg yolk immunoglobulins by oral delivery" @default.
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- W1497391707 doi "https://doi.org/10.1111/j.1365-2109.2010.02558.x" @default.
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