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- W1497393655 abstract "Abstract The template response of a purified DNA polymerase from regenerating rat liver has been studied. This enzyme does not copy a polyribonucleotide template; however, the ribo strand of a synthetic RNA-DNA hybrid can be used as an initiator for the synthesis of the complementary deoxyribo strand. This enzyme also has a marked preference for at least partially single stranded polydeoxyribonucleotide templates. The copolymer poly[d(A-T)·d(T-A)] is multiplied 4- or 8-fold. The single stranded poly(dA) acts as an efficient template for the regenerating rat liver DNA polymerase, provided short complementary oligodeoxyribonucleotide initiators are added. Poly(dC) is used as a template without added initiator. Synthesis of the complementary polydeoxyribonucleotide continues in both cases until an amount of polymer equal to the amount of initial template is produced. The enzyme does not copy poly(dG), either in a single stranded form in the presence of oligo(dC) or in a duplex form such as poly[(dC)·(dG)]. The specificities detected with synthetic polymers show that this DNA-dependent DNA polymerase is distinguishable from RNA-dependent DNA polymerases." @default.
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- W1497393655 date "1973-01-01" @default.
- W1497393655 modified "2023-10-18" @default.
- W1497393655 title "Synthetic Template Specificity of a Deoxyribonucleic Acid Polymerase from Regenerating Rat Liver" @default.
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- W1497393655 doi "https://doi.org/10.1016/s0021-9258(19)44454-2" @default.
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