FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1497413258> ?p ?o ?g. }

• W1497413258 endingPage "23006" @default.
• W1497413258 startingPage "23002" @default.
• W1497413258 abstract "To elucidate the interactions between residues found in the active-site cavity of human carbonic anhydrase III, we have prepared a series of single and double mutants with Lys-64, Arg-67, and Phe-198 replaced with Ala, Asp, Glu, His, and Leu. Rates of catalysis were determined using 18O exchange between CO2 and water measured by mass spectrometry and initial velocity measured by stopped-flow spectrophotometry. Replacement of these residues resulted in increases in kcat/Km for CO2 hydration as much as 200-fold and increases in the pKa of the zinc-bound water by as much as 3.5 units. We conclude that the effect of replacements made at positions 64, 67, and 198 were in general additive for kcat/Km for CO2 hydration, indicating that there is no interaction between these sites that affects the catalytic interconversion of CO2 and HCO3-. One notable exception is the antagonism exhibited by the double mutant of human carbonic anhydrase III containing Glu-64 and Leu-198. The data also show that one source of the large enhancement of kcat/Km for the mutant containing Asp-198 in human carbonic anhydrase III is the presence of both Asp-198 and Lys-64; when Lys-64 was replaced with Ala, a reduction of catalytic activity was observed. These results provide an additional view of the independent interactions of amino acids that affect the catalytic pathway of isozyme III, the least active of the known carbonic anhydrase isozymes." @default.
• W1497413258 created "2016-06-24" @default.
• W1497413258 creator A5004405545 @default.
• W1497413258 creator A5011625233 @default.
• W1497413258 creator A5034873616 @default.
• W1497413258 creator A5042083110 @default.
• W1497413258 creator A5057375922 @default.
• W1497413258 creator A5065140987 @default.
• W1497413258 creator A5083084332 @default.
• W1497413258 date "1994-09-01" @default.
• W1497413258 modified "2023-09-27" @default.
• W1497413258 title "Interactions of active-site residues and catalytic activity of human carbonic anhydrase III." @default.
• W1497413258 cites W1484765693 @default.
• W1497413258 cites W1498408076 @default.
• W1497413258 cites W1513087030 @default.
• W1497413258 cites W1551748714 @default.
• W1497413258 cites W1573899446 @default.
• W1497413258 cites W1585542991 @default.
• W1497413258 cites W1648076034 @default.
• W1497413258 cites W1873199501 @default.
• W1497413258 cites W1970259861 @default.
• W1497413258 cites W1972154782 @default.
• W1497413258 cites W1972548580 @default.
• W1497413258 cites W1974988689 @default.
• W1497413258 cites W1975193732 @default.
• W1497413258 cites W1976584967 @default.
• W1497413258 cites W1983563055 @default.
• W1497413258 cites W1988295340 @default.
• W1497413258 cites W1996343567 @default.
• W1497413258 cites W1998900102 @default.
• W1497413258 cites W2012132546 @default.
• W1497413258 cites W2016065841 @default.
• W1497413258 cites W2017229977 @default.
• W1497413258 cites W2019175962 @default.
• W1497413258 cites W2021667165 @default.
• W1497413258 cites W2028109808 @default.
• W1497413258 cites W2036005020 @default.
• W1497413258 cites W2038373026 @default.
• W1497413258 cites W2046801125 @default.
• W1497413258 cites W2049122844 @default.
• W1497413258 cites W2056476754 @default.
• W1497413258 cites W2060102273 @default.
• W1497413258 cites W2080785168 @default.
• W1497413258 cites W2084361872 @default.
• W1497413258 cites W2127116852 @default.
• W1497413258 doi "https://doi.org/10.1016/s0021-9258(17)31610-1" @default.
• W1497413258 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8083199" @default.
• W1497413258 hasPublicationYear "1994" @default.
• W1497413258 type Work @default.
• W1497413258 sameAs 1497413258 @default.
• W1497413258 citedByCount "13" @default.
• W1497413258 countsByYear W14974132582012 @default.
• W1497413258 countsByYear W14974132582014 @default.
• W1497413258 countsByYear W14974132582015 @default.
• W1497413258 countsByYear W14974132582017 @default.
• W1497413258 countsByYear W14974132582023 @default.
• W1497413258 crossrefType "journal-article" @default.
• W1497413258 hasAuthorship W1497413258A5004405545 @default.
• W1497413258 hasAuthorship W1497413258A5011625233 @default.
• W1497413258 hasAuthorship W1497413258A5034873616 @default.
• W1497413258 hasAuthorship W1497413258A5042083110 @default.
• W1497413258 hasAuthorship W1497413258A5057375922 @default.
• W1497413258 hasAuthorship W1497413258A5065140987 @default.
• W1497413258 hasAuthorship W1497413258A5083084332 @default.
• W1497413258 hasBestOaLocation W14974132581 @default.
• W1497413258 hasConcept C119795356 @default.
• W1497413258 hasConcept C161790260 @default.
• W1497413258 hasConcept C181199279 @default.
• W1497413258 hasConcept C185592680 @default.
• W1497413258 hasConcept C2775914622 @default.
• W1497413258 hasConcept C2780416268 @default.
• W1497413258 hasConcept C41183919 @default.
• W1497413258 hasConcept C55493867 @default.
• W1497413258 hasConcept C56856141 @default.
• W1497413258 hasConcept C71240020 @default.
• W1497413258 hasConceptScore W1497413258C119795356 @default.
• W1497413258 hasConceptScore W1497413258C161790260 @default.
• W1497413258 hasConceptScore W1497413258C181199279 @default.
• W1497413258 hasConceptScore W1497413258C185592680 @default.
• W1497413258 hasConceptScore W1497413258C2775914622 @default.
• W1497413258 hasConceptScore W1497413258C2780416268 @default.
• W1497413258 hasConceptScore W1497413258C41183919 @default.
• W1497413258 hasConceptScore W1497413258C55493867 @default.
• W1497413258 hasConceptScore W1497413258C56856141 @default.
• W1497413258 hasConceptScore W1497413258C71240020 @default.
• W1497413258 hasIssue "37" @default.
• W1497413258 hasLocation W14974132581 @default.
• W1497413258 hasOpenAccess W1497413258 @default.
• W1497413258 hasPrimaryLocation W14974132581 @default.
• W1497413258 hasRelatedWork W1519820833 @default.
• W1497413258 hasRelatedWork W1583827057 @default.
• W1497413258 hasRelatedWork W1589380177 @default.
• W1497413258 hasRelatedWork W1883722763 @default.
• W1497413258 hasRelatedWork W1969882477 @default.
• W1497413258 hasRelatedWork W2015280585 @default.
• W1497413258 hasRelatedWork W2015369153 @default.
• W1497413258 hasRelatedWork W2069584306 @default.
• W1497413258 hasRelatedWork W2477939661 @default.

• next