Matches in SemOpenAlex for { <https://semopenalex.org/work/W1497445092> ?p ?o ?g. }
Showing items 1 to 74 of
74
with 100 items per page.
- W1497445092 endingPage "3939" @default.
- W1497445092 startingPage "3933" @default.
- W1497445092 abstract "Abstract DNA-dependent RNA polymerase activity was extracted from nuclei of Dictyostelium discoideum at two stages in the life cycle: during exponential growth and during differentiation into fruiting bodies. The activity from both stages could be resolved into several fractions which differed in sensitivity to α-amanitin and in ionic requirements. No significant change in the proportions of the various fractions was found during development. Both polymerases I and II, isolated from either growing or differentiating cells, were found to prefer double-strand DNA of D. discoideum but could also use DNA from a variety of organisms to a lesser extent. No change in template specificity was observed during development. Polymerase II was purified to apparent homogeneity from both growing and differentiating cells and the subunit composition determined by acrylamide gel electrophoresis in the presence of dodecyl sulfate. Five protein subunits were consistently observed with approximate molecular weights of 170,000, 150,000, 28,00o, 21,000, and 15,000. Although a 190,000 molecular weight subunit was observed in certain preparations from growing cells, it was not found to be an obligatory component of the enzyme. These studies suggest that the change in the pattern of transcription which occurs during development in D. discoideum may depend on factors other than alteration of RNA polymerase." @default.
- W1497445092 created "2016-06-24" @default.
- W1497445092 creator A5014612742 @default.
- W1497445092 creator A5049593363 @default.
- W1497445092 date "1973-06-01" @default.
- W1497445092 modified "2023-09-30" @default.
- W1497445092 title "Multiple Nuclear Ribonucleic Acid Polymerases during Development of Dictyostelium discoideum" @default.
- W1497445092 cites W1524162190 @default.
- W1497445092 cites W1775749144 @default.
- W1497445092 cites W1893214469 @default.
- W1497445092 cites W1964491068 @default.
- W1497445092 cites W1969939375 @default.
- W1497445092 cites W1975011608 @default.
- W1497445092 cites W1994782699 @default.
- W1497445092 cites W2006201918 @default.
- W1497445092 cites W2014697803 @default.
- W1497445092 cites W2023367082 @default.
- W1497445092 cites W2024453791 @default.
- W1497445092 cites W2050050314 @default.
- W1497445092 cites W2078147272 @default.
- W1497445092 cites W2085268071 @default.
- W1497445092 cites W2090574291 @default.
- W1497445092 cites W2095254687 @default.
- W1497445092 cites W2100837269 @default.
- W1497445092 cites W2151945197 @default.
- W1497445092 doi "https://doi.org/10.1016/s0021-9258(19)43822-2" @default.
- W1497445092 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/4736080" @default.
- W1497445092 hasPublicationYear "1973" @default.
- W1497445092 type Work @default.
- W1497445092 sameAs 1497445092 @default.
- W1497445092 citedByCount "45" @default.
- W1497445092 crossrefType "journal-article" @default.
- W1497445092 hasAuthorship W1497445092A5014612742 @default.
- W1497445092 hasAuthorship W1497445092A5049593363 @default.
- W1497445092 hasBestOaLocation W14974450921 @default.
- W1497445092 hasConcept C104317684 @default.
- W1497445092 hasConcept C185592680 @default.
- W1497445092 hasConcept C2775969001 @default.
- W1497445092 hasConcept C552990157 @default.
- W1497445092 hasConcept C55493867 @default.
- W1497445092 hasConcept C67705224 @default.
- W1497445092 hasConcept C82381507 @default.
- W1497445092 hasConcept C86803240 @default.
- W1497445092 hasConcept C95444343 @default.
- W1497445092 hasConceptScore W1497445092C104317684 @default.
- W1497445092 hasConceptScore W1497445092C185592680 @default.
- W1497445092 hasConceptScore W1497445092C2775969001 @default.
- W1497445092 hasConceptScore W1497445092C552990157 @default.
- W1497445092 hasConceptScore W1497445092C55493867 @default.
- W1497445092 hasConceptScore W1497445092C67705224 @default.
- W1497445092 hasConceptScore W1497445092C82381507 @default.
- W1497445092 hasConceptScore W1497445092C86803240 @default.
- W1497445092 hasConceptScore W1497445092C95444343 @default.
- W1497445092 hasIssue "11" @default.
- W1497445092 hasLocation W14974450921 @default.
- W1497445092 hasOpenAccess W1497445092 @default.
- W1497445092 hasPrimaryLocation W14974450921 @default.
- W1497445092 hasRelatedWork W1848848102 @default.
- W1497445092 hasRelatedWork W1964562669 @default.
- W1497445092 hasRelatedWork W1975838976 @default.
- W1497445092 hasRelatedWork W1977222491 @default.
- W1497445092 hasRelatedWork W1977729177 @default.
- W1497445092 hasRelatedWork W1993079264 @default.
- W1497445092 hasRelatedWork W1996191277 @default.
- W1497445092 hasRelatedWork W2006250122 @default.
- W1497445092 hasRelatedWork W2743278168 @default.
- W1497445092 hasRelatedWork W4244201665 @default.
- W1497445092 hasVolume "248" @default.
- W1497445092 isParatext "false" @default.
- W1497445092 isRetracted "false" @default.
- W1497445092 magId "1497445092" @default.
- W1497445092 workType "article" @default.