FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1497452605> ?p ?o ?g. }

• W1497452605 endingPage "26524" @default.
• W1497452605 startingPage "26518" @default.
• W1497452605 abstract "A unique carboxyl proteinase (EC 3.4.23.33), insensitive to the classical inhibitor isovaleryl pepstatin and isolated from Pseudomonas sp. 101 (PCP), is the first example of a prokaryotic enzyme of this class. The gene coding for PCP was cloned, sequenced, and expressed in Escherichia coli. The gene consists of 1,761 base pairs encoding a protein of 587 amino acid residues. The NH2-terminal 215-amino acid preprosequence flanks the 372-amino acid mature protein, which is identical with the primary structure of an authentic PCP determined by chemical methods. E. coli carrying a plasmid containing the cloned wild-type PCP gene produced a 62-kDa protein. This molecule was processed and secreted into the periplasm as a 43-kDa protein, which converted to mature PCP under acidic conditions. This autocatalytic conversion was completely blocked by tyrostatin, a PCP-specific peptidic inhibitor from Kitasatosporia sp. 55. The purified recombinant PCP has the same characteristics as authentic PCP. When several preprosequence deletion mutants were expressed in E. coli, mutant proteins were accumulated as insoluble forms with no proteinase activities. These results suggest that the prepropeptide of PCP plays an essential role in the formation of functional PCP." @default.
• W1497452605 created "2016-06-24" @default.
• W1497452605 creator A5007968960 @default.
• W1497452605 creator A5015013402 @default.
• W1497452605 creator A5026581043 @default.
• W1497452605 creator A5030560624 @default.
• W1497452605 creator A5061174823 @default.
• W1497452605 date "1994-10-01" @default.
• W1497452605 modified "2023-10-18" @default.
• W1497452605 title "Cloning, nucleotide sequence, and expression of an isovaleryl pepstatin-insensitive carboxyl proteinase gene from Pseudomonas sp. 101" @default.
• W1497452605 cites W1512217822 @default.
• W1497452605 cites W1530466930 @default.
• W1497452605 cites W1530478933 @default.
• W1497452605 cites W1568285773 @default.
• W1497452605 cites W1581525501 @default.
• W1497452605 cites W1591438776 @default.
• W1497452605 cites W1612480077 @default.
• W1497452605 cites W1623705260 @default.
• W1497452605 cites W1761811505 @default.
• W1497452605 cites W1775749144 @default.
• W1497452605 cites W1907563531 @default.
• W1497452605 cites W1932052686 @default.
• W1497452605 cites W1965054762 @default.
• W1497452605 cites W1980333858 @default.
• W1497452605 cites W1991489244 @default.
• W1497452605 cites W2032462421 @default.
• W1497452605 cites W2047817844 @default.
• W1497452605 cites W2051813800 @default.
• W1497452605 cites W2061600981 @default.
• W1497452605 cites W2063450941 @default.
• W1497452605 cites W2100837269 @default.
• W1497452605 cites W2101108802 @default.
• W1497452605 cites W2129658625 @default.
• W1497452605 cites W2138270253 @default.
• W1497452605 cites W2166064969 @default.
• W1497452605 cites W2615372387 @default.
• W1497452605 doi "https://doi.org/10.1016/s0021-9258(18)47225-0" @default.
• W1497452605 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7929375" @default.
• W1497452605 hasPublicationYear "1994" @default.
• W1497452605 type Work @default.
• W1497452605 sameAs 1497452605 @default.
• W1497452605 citedByCount "47" @default.
• W1497452605 countsByYear W14974526052013 @default.
• W1497452605 countsByYear W14974526052017 @default.
• W1497452605 countsByYear W14974526052019 @default.
• W1497452605 countsByYear W14974526052022 @default.
• W1497452605 crossrefType "journal-article" @default.
• W1497452605 hasAuthorship W1497452605A5007968960 @default.
• W1497452605 hasAuthorship W1497452605A5015013402 @default.
• W1497452605 hasAuthorship W1497452605A5026581043 @default.
• W1497452605 hasAuthorship W1497452605A5030560624 @default.
• W1497452605 hasAuthorship W1497452605A5061174823 @default.
• W1497452605 hasBestOaLocation W14974526051 @default.
• W1497452605 hasConcept C104317684 @default.
• W1497452605 hasConcept C121050878 @default.
• W1497452605 hasConcept C153911025 @default.
• W1497452605 hasConcept C167625842 @default.
• W1497452605 hasConcept C181199279 @default.
• W1497452605 hasConcept C19924922 @default.
• W1497452605 hasConcept C199360897 @default.
• W1497452605 hasConcept C2776350231 @default.
• W1497452605 hasConcept C2776714187 @default.
• W1497452605 hasConcept C2778112365 @default.
• W1497452605 hasConcept C2781463711 @default.
• W1497452605 hasConcept C41008148 @default.
• W1497452605 hasConcept C523546767 @default.
• W1497452605 hasConcept C54355233 @default.
• W1497452605 hasConcept C55493867 @default.
• W1497452605 hasConcept C84148353 @default.
• W1497452605 hasConcept C86803240 @default.
• W1497452605 hasConceptScore W1497452605C104317684 @default.
• W1497452605 hasConceptScore W1497452605C121050878 @default.
• W1497452605 hasConceptScore W1497452605C153911025 @default.
• W1497452605 hasConceptScore W1497452605C167625842 @default.
• W1497452605 hasConceptScore W1497452605C181199279 @default.
• W1497452605 hasConceptScore W1497452605C19924922 @default.
• W1497452605 hasConceptScore W1497452605C199360897 @default.
• W1497452605 hasConceptScore W1497452605C2776350231 @default.
• W1497452605 hasConceptScore W1497452605C2776714187 @default.
• W1497452605 hasConceptScore W1497452605C2778112365 @default.
• W1497452605 hasConceptScore W1497452605C2781463711 @default.
• W1497452605 hasConceptScore W1497452605C41008148 @default.
• W1497452605 hasConceptScore W1497452605C523546767 @default.
• W1497452605 hasConceptScore W1497452605C54355233 @default.
• W1497452605 hasConceptScore W1497452605C55493867 @default.
• W1497452605 hasConceptScore W1497452605C84148353 @default.
• W1497452605 hasConceptScore W1497452605C86803240 @default.
• W1497452605 hasIssue "42" @default.
• W1497452605 hasLocation W14974526051 @default.
• W1497452605 hasOpenAccess W1497452605 @default.
• W1497452605 hasPrimaryLocation W14974526051 @default.
• W1497452605 hasRelatedWork W1497452605 @default.
• W1497452605 hasRelatedWork W1989391836 @default.
• W1497452605 hasRelatedWork W2043049374 @default.
• W1497452605 hasRelatedWork W2094660808 @default.
• W1497452605 hasRelatedWork W2349475057 @default.
• W1497452605 hasRelatedWork W2352588219 @default.
• W1497452605 hasRelatedWork W2355280460 @default.

• next