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• W1497487411 abstract "A cultured microalgal strain (UTEX 2540) originally identified as Heterotrichellagracillas Reisigl (Xanthophyceae) was re-examined using various techniques. Morphologicalevidence, particularly the absence of dimorphic cells (one blunt, the other tapered to an acutepoint), indicate that strain UTEX 2540 has been misidentified. Heterotrichella gracillas isconsidered to be a member of the chlorophyll a and c-containing class Xanthophyceae(Chromista). However, HPLC analyses of photosynthetic pigments indicated the presence ofchlorophylls a and b, s-carotene, lutein and violaxanthin while ultrastructural data revealed thepresence of starch stored inside the plastid. These data, as well as small subunit (18S rRNA)gene sequence analysis, indicate that this alga belongs in the Chlorophyta, not theXanthophyceae (Chromista). Further DNA sequence analyses suggest that UTEX 2540 is mostclosely related to Geminella terricola Petersen and certain Microspora species that are currentlyclassified in the Ulotrichales. However, unlike other Geminella species, UTEX 2540 exists assingle cells or forms poorly organized (2-8 celled) ephemeral pseudofilaments. A conspicuousextracellular mucilaginous sheath characterizes other Geminella species but this feature islacking in UTEX 2540. Furthermore, our analyses convincingly demonstrate that Geminella andat least some isolates of Microspora do not belong in the Ulotrichales. These results suggest that(1) the generic concept for Geminella must be broadened to include unicellular species that lackan apparent mucilaginous envelope, (2) Geminella does not belong in the Ulotrichales, and,instead, (3) its closest relatives among other green algae are almost certainly found within theTrebouxiophyceae." @default.
• W1497487411 created "2016-06-24" @default.
• W1497487411 creator A5047194342 @default.
• W1497487411 date "2009-01-01" @default.
• W1497487411 modified "2023-09-27" @default.
• W1497487411 title "A reassessment of Geminella (Chlorophyta) based upon photosynthetic pigments, DNA sequence analysis and electron microscopy" @default.
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