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- W1497683607 abstract "In X-ray microscopy different proteins are not readily distinguishable. However, in cell biology it is often desirable to localize single proteins, e.g., inside the cell nucleus. This can be achieved by immunogold labeling. Colloidal gold conjugated antibodies are used to mark the protein specifically. With silver solution these are enlarged so as to heighten their contrast. The strong absorption of silver allows easy visualization of the label in the nuclei. In this study male specific lethal 1 protein in male Drosophila melanogaster cells was labeled. This protein forms, together with four other proteins, a complex that is associated with the male X chromosome. It regulates dosage compensation by enhancing X-linked gene transcription in males. Room temperature and cyro transmission X-ray microscopic images (taken with the Göttingen TXM at BESSY) of these labeled cells are shown. Confocal laser scan microscopy ascertains the correct identification of the label in the X-ray micrographs, and allows comparison of the structural information available from both instruments." @default.
- W1497683607 created "2016-06-24" @default.
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- W1497683607 date "2000-01-01" @default.
- W1497683607 modified "2023-09-25" @default.
- W1497683607 title "X-ray microscopic studies of labeled nuclear cell structures" @default.
- W1497683607 doi "https://doi.org/10.1063/1.1291138" @default.
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