Matches in SemOpenAlex for { <https://semopenalex.org/work/W1497715321> ?p ?o ?g. }
Showing items 1 to 95 of
95
with 100 items per page.
- W1497715321 endingPage "853" @default.
- W1497715321 startingPage "846" @default.
- W1497715321 abstract "Several properties of the three acetohydroxy acid synthases of Escherichia coli have been compared in crude extracts. The three enzymes can be readily distinguished from each other. Acetohydroxy acid synthase I, the product of the ilvB gene, has been purified to near homogeneity. The purification was made possible by the fact that the enzyme was maintained in buffers of a high ionic strength or in buffers containing glycerol. Density gradient centrifugation studies indicated that the enzyme exists as a dimer of subunits of similar (60,000) molecular weight in buffers containing glycerol with or without two of the cofactors. Mg2+ and thiamine diphosphate. When flavine adenine dinucleotide was added along with Mg2+ and thiamine diphosphate, an increase in the rate of sedimentation occurred that was thought to be due to a rapid tetramer-dimer interconversion. The addition of pyruvate, the substrate, along with the three cofactors, resulted in a further increase in sedimentation rate, due presumably to an increase in the tetramer-to-dimer ratio. The addition of valine to the complete system resulted in maintenance of the enzyme in the dimeric state concomitant with inhibition of enzyme activity." @default.
- W1497715321 created "2016-06-24" @default.
- W1497715321 creator A5078339577 @default.
- W1497715321 creator A5085330137 @default.
- W1497715321 date "1979-02-01" @default.
- W1497715321 modified "2023-10-16" @default.
- W1497715321 title "Acetohydroxy acid synthase I of Escherichia coli: purification and properties" @default.
- W1497715321 cites W145448486 @default.
- W1497715321 cites W1484115349 @default.
- W1497715321 cites W1507782220 @default.
- W1497715321 cites W1517674875 @default.
- W1497715321 cites W1611482599 @default.
- W1497715321 cites W1625660792 @default.
- W1497715321 cites W184664806 @default.
- W1497715321 cites W1849997836 @default.
- W1497715321 cites W187916492 @default.
- W1497715321 cites W1905539396 @default.
- W1497715321 cites W1964756865 @default.
- W1497715321 cites W1998776758 @default.
- W1497715321 cites W2003555687 @default.
- W1497715321 cites W2004326208 @default.
- W1497715321 cites W2041742043 @default.
- W1497715321 cites W2064724755 @default.
- W1497715321 cites W2075828700 @default.
- W1497715321 cites W2090330769 @default.
- W1497715321 cites W2111301002 @default.
- W1497715321 cites W2122877741 @default.
- W1497715321 cites W2313928339 @default.
- W1497715321 cites W29931780 @default.
- W1497715321 doi "https://doi.org/10.1128/jb.137.2.846-853.1979" @default.
- W1497715321 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/218366" @default.
- W1497715321 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/370104" @default.
- W1497715321 hasPublicationYear "1979" @default.
- W1497715321 type Work @default.
- W1497715321 sameAs 1497715321 @default.
- W1497715321 citedByCount "78" @default.
- W1497715321 countsByYear W14977153212012 @default.
- W1497715321 countsByYear W14977153212014 @default.
- W1497715321 countsByYear W14977153212015 @default.
- W1497715321 countsByYear W14977153212016 @default.
- W1497715321 countsByYear W14977153212017 @default.
- W1497715321 countsByYear W14977153212021 @default.
- W1497715321 crossrefType "journal-article" @default.
- W1497715321 hasAuthorship W1497715321A5078339577 @default.
- W1497715321 hasAuthorship W1497715321A5085330137 @default.
- W1497715321 hasBestOaLocation W14977153211 @default.
- W1497715321 hasConcept C104317684 @default.
- W1497715321 hasConcept C178790620 @default.
- W1497715321 hasConcept C181199279 @default.
- W1497715321 hasConcept C185592680 @default.
- W1497715321 hasConcept C197957613 @default.
- W1497715321 hasConcept C2775859393 @default.
- W1497715321 hasConcept C2778886173 @default.
- W1497715321 hasConcept C2779546866 @default.
- W1497715321 hasConcept C2780047943 @default.
- W1497715321 hasConcept C33093398 @default.
- W1497715321 hasConcept C547475151 @default.
- W1497715321 hasConcept C55493867 @default.
- W1497715321 hasConcept C86803240 @default.
- W1497715321 hasConceptScore W1497715321C104317684 @default.
- W1497715321 hasConceptScore W1497715321C178790620 @default.
- W1497715321 hasConceptScore W1497715321C181199279 @default.
- W1497715321 hasConceptScore W1497715321C185592680 @default.
- W1497715321 hasConceptScore W1497715321C197957613 @default.
- W1497715321 hasConceptScore W1497715321C2775859393 @default.
- W1497715321 hasConceptScore W1497715321C2778886173 @default.
- W1497715321 hasConceptScore W1497715321C2779546866 @default.
- W1497715321 hasConceptScore W1497715321C2780047943 @default.
- W1497715321 hasConceptScore W1497715321C33093398 @default.
- W1497715321 hasConceptScore W1497715321C547475151 @default.
- W1497715321 hasConceptScore W1497715321C55493867 @default.
- W1497715321 hasConceptScore W1497715321C86803240 @default.
- W1497715321 hasIssue "2" @default.
- W1497715321 hasLocation W14977153211 @default.
- W1497715321 hasLocation W14977153212 @default.
- W1497715321 hasLocation W14977153213 @default.
- W1497715321 hasOpenAccess W1497715321 @default.
- W1497715321 hasPrimaryLocation W14977153211 @default.
- W1497715321 hasRelatedWork W1964468778 @default.
- W1497715321 hasRelatedWork W1975646510 @default.
- W1497715321 hasRelatedWork W1987636796 @default.
- W1497715321 hasRelatedWork W2003889801 @default.
- W1497715321 hasRelatedWork W2025398173 @default.
- W1497715321 hasRelatedWork W2079006240 @default.
- W1497715321 hasRelatedWork W2091146030 @default.
- W1497715321 hasRelatedWork W2098689981 @default.
- W1497715321 hasRelatedWork W2162473589 @default.
- W1497715321 hasRelatedWork W2414777250 @default.
- W1497715321 hasVolume "137" @default.
- W1497715321 isParatext "false" @default.
- W1497715321 isRetracted "false" @default.
- W1497715321 magId "1497715321" @default.
- W1497715321 workType "article" @default.