FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1498016263> ?p ?o ?g. }

• W1498016263 endingPage "31968" @default.
• W1498016263 startingPage "31962" @default.
• W1498016263 abstract "The splicing activities of 5-fluorouracil (FUra)-substituted U2 and U6 small nuclear RNAs (snRNAs) were examined in an in vitro splicing system. Yeast splicing extracts were specifically depleted of endogenous U2 and U6 snRNAs by antisense oligonucleotide-directed RNase H hydrolysis. Splicing activity was recovered when the extracts were reconstituted with synthetic U2 and U6 snRNAs. However, U2 snRNA with all uracils substituted with FUra (FU2) did not restore any splicing activity. Nondenaturing gel electrophoresis showed that FU2 failed to promote the assembly of spliceosome complexes. The ability of U2 snRNA to restore splicing in U2-depleted extracts increased as FUra content decreased but was still only 60% of control activity at 25% substitution of uracils with FUra. Addition of FU2 to nondepleted extracts caused strong inhibition of splicing accompanied by increased degradation of the pre-mRNA, suggesting that FU2 forms an inactive complex with a protein splicing factor that normally binds to the pre-mRNA. FU6 restored full splicing activity to U6-depleted extracts, but at a 5-fold higher concentration than U6 snRNA. These results demonstrate that the incorporation of FUra can impair the functions of catalytic RNA molecules." @default.
• W1498016263 created "2016-06-24" @default.
• W1498016263 creator A5029279649 @default.
• W1498016263 creator A5045441776 @default.
• W1498016263 creator A5069289875 @default.
• W1498016263 creator A5071711204 @default.
• W1498016263 date "1994-12-01" @default.
• W1498016263 modified "2023-10-17" @default.
• W1498016263 title "Incorporation of 5-fluorouracil into U2 and U6 snRNA inhibits mRNA precursor splicing." @default.
• W1498016263 cites W1507006251 @default.
• W1498016263 cites W1513879319 @default.
• W1498016263 cites W1516160268 @default.
• W1498016263 cites W1526030514 @default.
• W1498016263 cites W1556474484 @default.
• W1498016263 cites W1578318816 @default.
• W1498016263 cites W1584389037 @default.
• W1498016263 cites W1591000486 @default.
• W1498016263 cites W1604012490 @default.
• W1498016263 cites W1967900723 @default.
• W1498016263 cites W1968400447 @default.
• W1498016263 cites W1972322299 @default.
• W1498016263 cites W1974630548 @default.
• W1498016263 cites W1979455742 @default.
• W1498016263 cites W1980424685 @default.
• W1498016263 cites W1988271866 @default.
• W1498016263 cites W2006105381 @default.
• W1498016263 cites W2015445088 @default.
• W1498016263 cites W2022176376 @default.
• W1498016263 cites W2024293717 @default.
• W1498016263 cites W2035100616 @default.
• W1498016263 cites W2055492153 @default.
• W1498016263 cites W2055644037 @default.
• W1498016263 cites W2071032973 @default.
• W1498016263 cites W2075362101 @default.
• W1498016263 cites W2087137984 @default.
• W1498016263 cites W2089977049 @default.
• W1498016263 cites W2114992132 @default.
• W1498016263 cites W2127584949 @default.
• W1498016263 cites W2141451386 @default.
• W1498016263 cites W4237655687 @default.
• W1498016263 cites W4246499043 @default.
• W1498016263 cites W4379410178 @default.
• W1498016263 doi "https://doi.org/10.1016/s0021-9258(18)31789-7" @default.
• W1498016263 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7989372" @default.
• W1498016263 hasPublicationYear "1994" @default.
• W1498016263 type Work @default.
• W1498016263 sameAs 1498016263 @default.
• W1498016263 citedByCount "32" @default.
• W1498016263 countsByYear W14980162632013 @default.
• W1498016263 countsByYear W14980162632015 @default.
• W1498016263 countsByYear W14980162632020 @default.
• W1498016263 crossrefType "journal-article" @default.
• W1498016263 hasAuthorship W1498016263A5029279649 @default.
• W1498016263 hasAuthorship W1498016263A5045441776 @default.
• W1498016263 hasAuthorship W1498016263A5069289875 @default.
• W1498016263 hasAuthorship W1498016263A5071711204 @default.
• W1498016263 hasBestOaLocation W14980162631 @default.
• W1498016263 hasConcept C100299639 @default.
• W1498016263 hasConcept C102063271 @default.
• W1498016263 hasConcept C104317684 @default.
• W1498016263 hasConcept C105580179 @default.
• W1498016263 hasConcept C121608353 @default.
• W1498016263 hasConcept C185592680 @default.
• W1498016263 hasConcept C194993378 @default.
• W1498016263 hasConcept C2780456651 @default.
• W1498016263 hasConcept C54355233 @default.
• W1498016263 hasConcept C54458228 @default.
• W1498016263 hasConcept C55493867 @default.
• W1498016263 hasConcept C67705224 @default.
• W1498016263 hasConcept C86803240 @default.
• W1498016263 hasConcept C95444343 @default.
• W1498016263 hasConceptScore W1498016263C100299639 @default.
• W1498016263 hasConceptScore W1498016263C102063271 @default.
• W1498016263 hasConceptScore W1498016263C104317684 @default.
• W1498016263 hasConceptScore W1498016263C105580179 @default.
• W1498016263 hasConceptScore W1498016263C121608353 @default.
• W1498016263 hasConceptScore W1498016263C185592680 @default.
• W1498016263 hasConceptScore W1498016263C194993378 @default.
• W1498016263 hasConceptScore W1498016263C2780456651 @default.
• W1498016263 hasConceptScore W1498016263C54355233 @default.
• W1498016263 hasConceptScore W1498016263C54458228 @default.
• W1498016263 hasConceptScore W1498016263C55493867 @default.
• W1498016263 hasConceptScore W1498016263C67705224 @default.
• W1498016263 hasConceptScore W1498016263C86803240 @default.
• W1498016263 hasConceptScore W1498016263C95444343 @default.
• W1498016263 hasIssue "50" @default.
• W1498016263 hasLocation W14980162631 @default.
• W1498016263 hasOpenAccess W1498016263 @default.
• W1498016263 hasPrimaryLocation W14980162631 @default.
• W1498016263 hasRelatedWork W1499027396 @default.
• W1498016263 hasRelatedWork W1998544049 @default.
• W1498016263 hasRelatedWork W2004746002 @default.
• W1498016263 hasRelatedWork W2014751751 @default.
• W1498016263 hasRelatedWork W2062011059 @default.
• W1498016263 hasRelatedWork W2091106905 @default.
• W1498016263 hasRelatedWork W2113243883 @default.
• W1498016263 hasRelatedWork W2126414543 @default.
• W1498016263 hasRelatedWork W2632544498 @default.

• next