FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1498146861> ?p ?o ?g. }

• W1498146861 endingPage "12812" @default.
• W1498146861 startingPage "12809" @default.
• W1498146861 abstract "To evaluate the synthesis and initial processing of the insulin receptor precursor, we compared cell-free translation of rat liver poly(A)+ RNA in a reticulocyte lysate system with metabolically labeled rat hepatoma (Fao) cells. In in vitro translation assays, the primary L-[35S]cysteine-labeled products of rat liver mRNA specifically immunoprecipitable with insulin receptor antiserum were two closely migrating polypeptides with a Mr range of 160,000-164,000 (n = 7). This is similar to the size predicted by the insulin receptor cDNA sequence. When heterologous microsomal membranes were included in the cell-free system to process newly synthesized proteins co-translationally, the receptor precursors migrated as larger species of 180 +/- 2 kDa (n = 3). For comparison, when Fao cells were treated with tunicamycin to block core N-glycosylation and pulse-labeled with L-[35S]methionine, two closely migrating precursors were labeled that co-migrated with the unprocessed in vitro translation products (approximately 160 kDa). Pulse labeling of Fao cells in the absence of tunicamycin revealed receptor precursor species of 188 and 198 kDa that rapidly disappeared (t1/2 = 54 min) as the receptor subunits were observed. Thus, the initial products of insulin receptor mRNA translation are two approximately 162-kDa polypeptides that are rapidly processed in intact cells and can only be observed by in vitro studies or by using inhibitors of core glycosylation. Insulin proreceptor species can also be partially glycosylated during cell-free translation by added microsomal membranes. This is the first description of cell-free translation of the insulin proreceptor in a system that will allow detailed characterization of the earliest steps in insulin receptor biogenesis." @default.
• W1498146861 created "2016-06-24" @default.
• W1498146861 creator A5001485785 @default.
• W1498146861 creator A5051737440 @default.
• W1498146861 date "1988-09-01" @default.
• W1498146861 modified "2023-10-14" @default.
• W1498146861 title "Initial processing of the insulin receptor precursor in vivo and in vitro." @default.
• W1498146861 cites W1412217579 @default.
• W1498146861 cites W1490786462 @default.
• W1498146861 cites W1494794695 @default.
• W1498146861 cites W1497023230 @default.
• W1498146861 cites W1560338125 @default.
• W1498146861 cites W1584935191 @default.
• W1498146861 cites W1599848121 @default.
• W1498146861 cites W1985857745 @default.
• W1498146861 cites W1987618777 @default.
• W1498146861 cites W1989825490 @default.
• W1498146861 cites W1991710558 @default.
• W1498146861 cites W2002830209 @default.
• W1498146861 cites W2026533116 @default.
• W1498146861 cites W2037574540 @default.
• W1498146861 cites W2062198383 @default.
• W1498146861 cites W2066160869 @default.
• W1498146861 cites W2071799329 @default.
• W1498146861 cites W2075558536 @default.
• W1498146861 cites W2076752280 @default.
• W1498146861 cites W2083643026 @default.
• W1498146861 cites W2085688177 @default.
• W1498146861 cites W2087995258 @default.
• W1498146861 cites W2100837269 @default.
• W1498146861 cites W2106172355 @default.
• W1498146861 cites W2122567567 @default.
• W1498146861 cites W2176848385 @default.
• W1498146861 cites W2340811073 @default.
• W1498146861 cites W2462096058 @default.
• W1498146861 doi "https://doi.org/10.1016/s0021-9258(18)37629-4" @default.
• W1498146861 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/3417632" @default.
• W1498146861 hasPublicationYear "1988" @default.
• W1498146861 type Work @default.
• W1498146861 sameAs 1498146861 @default.
• W1498146861 citedByCount "11" @default.
• W1498146861 countsByYear W14981468612020 @default.
• W1498146861 crossrefType "journal-article" @default.
• W1498146861 hasAuthorship W1498146861A5001485785 @default.
• W1498146861 hasAuthorship W1498146861A5051737440 @default.
• W1498146861 hasBestOaLocation W14981468611 @default.
• W1498146861 hasConcept C112446052 @default.
• W1498146861 hasConcept C126322002 @default.
• W1498146861 hasConcept C134018914 @default.
• W1498146861 hasConcept C150903083 @default.
• W1498146861 hasConcept C170493617 @default.
• W1498146861 hasConcept C185592680 @default.
• W1498146861 hasConcept C202751555 @default.
• W1498146861 hasConcept C207001950 @default.
• W1498146861 hasConcept C2777391703 @default.
• W1498146861 hasConcept C2779306644 @default.
• W1498146861 hasConcept C55493867 @default.
• W1498146861 hasConcept C71924100 @default.
• W1498146861 hasConcept C86803240 @default.
• W1498146861 hasConcept C95444343 @default.
• W1498146861 hasConceptScore W1498146861C112446052 @default.
• W1498146861 hasConceptScore W1498146861C126322002 @default.
• W1498146861 hasConceptScore W1498146861C134018914 @default.
• W1498146861 hasConceptScore W1498146861C150903083 @default.
• W1498146861 hasConceptScore W1498146861C170493617 @default.
• W1498146861 hasConceptScore W1498146861C185592680 @default.
• W1498146861 hasConceptScore W1498146861C202751555 @default.
• W1498146861 hasConceptScore W1498146861C207001950 @default.
• W1498146861 hasConceptScore W1498146861C2777391703 @default.
• W1498146861 hasConceptScore W1498146861C2779306644 @default.
• W1498146861 hasConceptScore W1498146861C55493867 @default.
• W1498146861 hasConceptScore W1498146861C71924100 @default.
• W1498146861 hasConceptScore W1498146861C86803240 @default.
• W1498146861 hasConceptScore W1498146861C95444343 @default.
• W1498146861 hasIssue "26" @default.
• W1498146861 hasLocation W14981468611 @default.
• W1498146861 hasOpenAccess W1498146861 @default.
• W1498146861 hasPrimaryLocation W14981468611 @default.
• W1498146861 hasRelatedWork W1983641721 @default.
• W1498146861 hasRelatedWork W1996604497 @default.
• W1498146861 hasRelatedWork W2010140898 @default.
• W1498146861 hasRelatedWork W2085082785 @default.
• W1498146861 hasRelatedWork W2098842401 @default.
• W1498146861 hasRelatedWork W2118367091 @default.
• W1498146861 hasRelatedWork W2135403984 @default.
• W1498146861 hasRelatedWork W2149250249 @default.
• W1498146861 hasRelatedWork W2150233632 @default.
• W1498146861 hasRelatedWork W4234612679 @default.
• W1498146861 hasVolume "263" @default.
• W1498146861 isParatext "false" @default.
• W1498146861 isRetracted "false" @default.
• W1498146861 magId "1498146861" @default.
• W1498146861 workType "article" @default.