FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1498183721> ?p ?o ?g. }

• W1498183721 endingPage "9121" @default.
• W1498183721 startingPage "9118" @default.
• W1498183721 abstract "Abstract Bacterial lipopolysaccharide (LPS) potentiates protein kinase C (PKC)-dependent responses such as the activation of arachidonic acid metabolism in macrophages (Aderem, A. A., Cohen, D. S., Wright, S. D., and Cohn, Z. A. (1986) J. Exp. Med. 164, 165-179). Concomitantly, LPS promotes the myristoylation of a 68K PKC substrate, shown to be equivalent to the 80/87K PKC substrate found in brain and fibroblasts (Aderem, A. A., Albert, K. A., Keum, M. M., Wang, J. K., Greengard, P., and Cohn, Z. A. (1988) Nature 332, 362-364). We have now examined the effect of LPS on the phosphorylation of this 68K PKC substrate. We report here that LPS modifies the kinetics and extent of phosphorylation of the 68K protein. While treatment with LPS alone induces low level phosphorylation of the 68K protein, it markedly increases the rate of subsequent phorbol 12-myristate 13-acetate (PMA)-dependent phosphorylation of this protein. Phosphorylation in LPS-treated macrophages was maximal 1-2 min after administration of PMA, while maximal phosphorylation in macrophages not exposed to LPS was only achieved 6 min after addition of PMA. In addition to increasing the rate of PMA-dependent phosphorylation of the 68K protein in macrophages, LPS also promoted the phosphorylation of a novel peptide on the 68K protein. Thus while PMA stimulated the phosphorylation of two thermolytic phosphopeptides (phosphopeptides 1 and 2), the low level of phosphorylation observed with LPS alone was found to occur on phosphopeptides 1 and 2 as well as on a novel phosphopeptide (phosphopeptide 3). Furthermore, LPS treatment of macrophages potentiated phosphorylation of all three phosphopeptides when the cells were subsequently stimulated with PMA. While phosphorylation stimulated by LPS and PMA was slightly more than additive for phosphopeptides 1 and 2, it was markedly synergistic (increased 14.5-fold) for phosphopeptide 3. Phosphorylation of all three phosphopeptides occurred exclusively on serine. It is possible that LPS-induced myristoylation of the 68K protein directs it to the membrane where its phosphorylation is enhanced by its close association with PKC." @default.
• W1498183721 created "2016-06-24" @default.
• W1498183721 creator A5008833389 @default.
• W1498183721 creator A5021509115 @default.
• W1498183721 creator A5047708722 @default.
• W1498183721 creator A5052825834 @default.
• W1498183721 creator A5063227404 @default.
• W1498183721 date "1989-06-01" @default.
• W1498183721 modified "2023-10-13" @default.
• W1498183721 title "Bacterial Lipopolysaccharide Regulates the Phosphorylation of the 68K Protein Kinase C Substrate in Macrophages" @default.
• W1498183721 cites W131448777 @default.
• W1498183721 cites W1480686961 @default.
• W1498183721 cites W1529922998 @default.
• W1498183721 cites W1569108584 @default.
• W1498183721 cites W1583292806 @default.
• W1498183721 cites W1597762117 @default.
• W1498183721 cites W1964839077 @default.
• W1498183721 cites W1973261169 @default.
• W1498183721 cites W1976329278 @default.
• W1498183721 cites W1993282564 @default.
• W1498183721 cites W2027023788 @default.
• W1498183721 cites W2029374595 @default.
• W1498183721 cites W2066768102 @default.
• W1498183721 cites W2080871752 @default.
• W1498183721 cites W2085216298 @default.
• W1498183721 cites W2100837269 @default.
• W1498183721 doi "https://doi.org/10.1016/s0021-9258(18)60499-5" @default.
• W1498183721 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2722820" @default.
• W1498183721 hasPublicationYear "1989" @default.
• W1498183721 type Work @default.
• W1498183721 sameAs 1498183721 @default.
• W1498183721 citedByCount "59" @default.
• W1498183721 countsByYear W14981837212012 @default.
• W1498183721 countsByYear W14981837212013 @default.
• W1498183721 crossrefType "journal-article" @default.
• W1498183721 hasAuthorship W1498183721A5008833389 @default.
• W1498183721 hasAuthorship W1498183721A5021509115 @default.
• W1498183721 hasAuthorship W1498183721A5047708722 @default.
• W1498183721 hasAuthorship W1498183721A5052825834 @default.
• W1498183721 hasAuthorship W1498183721A5063227404 @default.
• W1498183721 hasBestOaLocation W14981837211 @default.
• W1498183721 hasConcept C11960822 @default.
• W1498183721 hasConcept C173990801 @default.
• W1498183721 hasConcept C184235292 @default.
• W1498183721 hasConcept C185592680 @default.
• W1498183721 hasConcept C18903297 @default.
• W1498183721 hasConcept C203014093 @default.
• W1498183721 hasConcept C2777289219 @default.
• W1498183721 hasConcept C2778754761 @default.
• W1498183721 hasConcept C55493867 @default.
• W1498183721 hasConcept C86803240 @default.
• W1498183721 hasConcept C89423630 @default.
• W1498183721 hasConcept C95444343 @default.
• W1498183721 hasConceptScore W1498183721C11960822 @default.
• W1498183721 hasConceptScore W1498183721C173990801 @default.
• W1498183721 hasConceptScore W1498183721C184235292 @default.
• W1498183721 hasConceptScore W1498183721C185592680 @default.
• W1498183721 hasConceptScore W1498183721C18903297 @default.
• W1498183721 hasConceptScore W1498183721C203014093 @default.
• W1498183721 hasConceptScore W1498183721C2777289219 @default.
• W1498183721 hasConceptScore W1498183721C2778754761 @default.
• W1498183721 hasConceptScore W1498183721C55493867 @default.
• W1498183721 hasConceptScore W1498183721C86803240 @default.
• W1498183721 hasConceptScore W1498183721C89423630 @default.
• W1498183721 hasConceptScore W1498183721C95444343 @default.
• W1498183721 hasIssue "16" @default.
• W1498183721 hasLocation W14981837211 @default.
• W1498183721 hasOpenAccess W1498183721 @default.
• W1498183721 hasPrimaryLocation W14981837211 @default.
• W1498183721 hasRelatedWork W1964515703 @default.
• W1498183721 hasRelatedWork W1978425204 @default.
• W1498183721 hasRelatedWork W1999792639 @default.
• W1498183721 hasRelatedWork W2029161345 @default.
• W1498183721 hasRelatedWork W2051108283 @default.
• W1498183721 hasRelatedWork W2056603697 @default.
• W1498183721 hasRelatedWork W2127013244 @default.
• W1498183721 hasRelatedWork W3199639092 @default.
• W1498183721 hasRelatedWork W4362610326 @default.
• W1498183721 hasRelatedWork W4362623556 @default.
• W1498183721 hasVolume "264" @default.
• W1498183721 isParatext "false" @default.
• W1498183721 isRetracted "false" @default.
• W1498183721 magId "1498183721" @default.
• W1498183721 workType "article" @default.