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• W1498210985 abstract "DNA sequences are now far more readily available in silico than as physical DNA. De novo gene synthesis is an increasingly cost-effective method for building genetic constructs, and effectively removes the constraint of basing constructs on extant sequences. This allows scientists and engineers to experimentally test their hypotheses relating sequence to function. Molecular biologists, and now synthetic biologists, are characterizing and cataloging genetic elements with specific functions, aiming to combine them to perform complex functions. However, the most common purpose of synthetic genes is for the expression of an encoded protein. The huge number of different proteins makes it impossible to characterize and catalog each functional gene. Instead, it is necessary to abstract design principles from experimental data: data that can be generated by making predictions followed by synthesizing sequences to test those predictions. Because of the degeneracy of the genetic code, design of gene sequences to encode proteins is a high-dimensional problem, so there is no single simple formula to guarantee success. Nevertheless, there are several straightforward steps that can be taken to greatly increase the probability that a designed sequence will result in expression of the encoded protein. In this chapter, we discuss gene sequence parameters that are important for protein expression. We also describe algorithms for optimizing these parameters, and troubleshooting procedures that can be helpful when initial attempts fail. Finally, we show how many of these methods can be accomplished using the synthetic biology software tool Gene Designer." @default.
• W1498210985 created "2016-06-24" @default.
• W1498210985 creator A5011783279 @default.
• W1498210985 creator A5047966663 @default.
• W1498210985 creator A5078447171 @default.
• W1498210985 creator A5088443217 @default.
• W1498210985 date "2011-01-01" @default.
• W1498210985 modified "2023-10-08" @default.
• W1498210985 title "Designing Genes for Successful Protein Expression" @default.
• W1498210985 cites W1692054786 @default.
• W1498210985 cites W1972630690 @default.
• W1498210985 cites W1973910583 @default.
• W1498210985 cites W1974677665 @default.
• W1498210985 cites W1978885095 @default.
• W1498210985 cites W1983761574 @default.
• W1498210985 cites W1983849957 @default.
• W1498210985 cites W1986493208 @default.
• W1498210985 cites W1992404702 @default.
• W1498210985 cites W2004079991 @default.
• W1498210985 cites W2004133790 @default.
• W1498210985 cites W2011828065 @default.
• W1498210985 cites W2013642367 @default.
• W1498210985 cites W2014177423 @default.
• W1498210985 cites W2018147562 @default.
• W1498210985 cites W2018590530 @default.
• W1498210985 cites W2019337061 @default.
• W1498210985 cites W2021842042 @default.
• W1498210985 cites W2028165934 @default.
• W1498210985 cites W2030799825 @default.
• W1498210985 cites W2031853427 @default.
• W1498210985 cites W2032429123 @default.
• W1498210985 cites W2039756922 @default.
• W1498210985 cites W2042700625 @default.
• W1498210985 cites W2043335299 @default.
• W1498210985 cites W2047360542 @default.
• W1498210985 cites W2048003108 @default.
• W1498210985 cites W2051242197 @default.
• W1498210985 cites W2051482716 @default.
• W1498210985 cites W2054261154 @default.
• W1498210985 cites W2055427321 @default.
• W1498210985 cites W2057814775 @default.
• W1498210985 cites W2058268622 @default.
• W1498210985 cites W2058426472 @default.
• W1498210985 cites W2058451208 @default.
• W1498210985 cites W2062117958 @default.
• W1498210985 cites W2063779182 @default.
• W1498210985 cites W2066837313 @default.
• W1498210985 cites W2069455029 @default.
• W1498210985 cites W2072499238 @default.
• W1498210985 cites W2072971665 @default.
• W1498210985 cites W2076058857 @default.
• W1498210985 cites W2076709958 @default.
• W1498210985 cites W2077425084 @default.
• W1498210985 cites W2078755055 @default.
• W1498210985 cites W2079136072 @default.
• W1498210985 cites W2079148256 @default.
• W1498210985 cites W2083842949 @default.
• W1498210985 cites W2086344973 @default.
• W1498210985 cites W2089165095 @default.
• W1498210985 cites W2095675850 @default.
• W1498210985 cites W2096186501 @default.
• W1498210985 cites W2097775940 @default.
• W1498210985 cites W2099435024 @default.
• W1498210985 cites W2100100451 @default.
• W1498210985 cites W2100758875 @default.
• W1498210985 cites W2101918141 @default.
• W1498210985 cites W2104295567 @default.
• W1498210985 cites W2108329085 @default.
• W1498210985 cites W2110705025 @default.
• W1498210985 cites W2112902581 @default.
• W1498210985 cites W2114900973 @default.
• W1498210985 cites W2116541947 @default.
• W1498210985 cites W2117593281 @default.
• W1498210985 cites W2119336718 @default.
• W1498210985 cites W2122692349 @default.
• W1498210985 cites W2132963003 @default.
• W1498210985 cites W2134398014 @default.
• W1498210985 cites W2138721124 @default.
• W1498210985 cites W2140486583 @default.
• W1498210985 cites W2141820518 @default.
• W1498210985 cites W2143221412 @default.
• W1498210985 cites W2147982990 @default.
• W1498210985 cites W2153784832 @default.
• W1498210985 cites W2154140542 @default.
• W1498210985 cites W2155888222 @default.
• W1498210985 cites W2156536014 @default.
• W1498210985 cites W2156628986 @default.
• W1498210985 cites W2162430545 @default.
• W1498210985 cites W2164754533 @default.
• W1498210985 cites W2170602689 @default.
• W1498210985 cites W2170667639 @default.
• W1498210985 cites W2395549144 @default.
• W1498210985 cites W4240480203 @default.
• W1498210985 doi "https://doi.org/10.1016/b978-0-12-385120-8.00003-6" @default.
• W1498210985 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21601673" @default.
• W1498210985 hasPublicationYear "2011" @default.
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