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• W1498387507 abstract "The effluent from a narrow-bore liquid chromatographic (l.c.) separation can be immobilized on thin-layer chromatographic (t.l.c.) plates with little loss of resolution. The deposited compounds are then available for further inspection. For off-line detection, direct fluorescence emission, fluorescence excitation emission spectra, and fluorescence line-narrowing spectroscopy are investigated with tetracene and benz[k] fluoranthene as model compounds. Detection based on direct emission measurements and on measurements for which complete spectra are obtained for the separated compounds, is suitable for identification and determination. Detection limits are of the same order of magnitude as those for on-line detection in narrow-bore l.c. The fluorescence spectra of immobilized compounds can be obtained with a conventional fluorescence spectrometer equipped with a solid-sample accessory. No other special apparatus is needed. The immobilized chromatogram is also suitable for techniques incompatible with flow systems, e.g., fluorescence line-narrowing spectroscopy, which yields fluorescence spectra via laser excitation of low-temperature solid samples. Very selective narrow-line fluorescence spectra were obtained for tetracene deposited on t.l.c. plates in amounts down to the low picogram level." @default.
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• W1498387507 date "1987-01-01" @default.
• W1498387507 modified "2023-09-26" @default.
• W1498387507 title "Coupling of narrow-bore liquid chromatography to thin-layer chromatography" @default.
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• W1498387507 doi "https://doi.org/10.1016/s0003-2670(00)86151-6" @default.
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