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- W1498389547 abstract "Human peripheral blood mononuclear cells, B-cell-enriched populations, and T cells were separated according to their buoyant densities using discontinuous gradients of Percoll. Lymphocyte subpopulations were stimulated with various mitogens and polyclonal activators. Low-density T-cell fractions responded better than total T cells or high-density fractions when stimulated with phytohemagglutinin, concanavalin A, and pokeweed mitogen. A low-density B-cell fraction differentiated better than other fractions after stimulation with PWM and total T cells, while high-density B cells (with or without T cells) gave the greatest plasma cell response when treated with lipopolysaccharide. Differential contamination of individual fractions by monocytes do not explain these findings. Thus, both T- and B-lymphocyte subpopulations can be separated by their buoyant densities into subpopulations displaying distinct reactivities toward mitogens and polyclonal activators." @default.
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- W1498389547 date "1981-10-01" @default.
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- W1498389547 title "Human B-lymphocyte subpopulations" @default.
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- W1498389547 doi "https://doi.org/10.1016/0090-1229(81)90193-8" @default.
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