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- W1498454975 abstract "Publisher Summary This chapter describes the experimental protocols underlying the process of activation tagging. The chapter focuses on the use of this experimental approach to generate mutant cell (and plant) lines able to grow in culture in the absence of auxin, and discusses the general use of this method of tagging to isolate genes whose overexpression results in a selectable phenotype. The creation of dominant mutations through transcriptional activation provides two immediate advantages over other strategies of gene tagging. First, it allows the direct selection for a specific phenotype from among the population of primary transformants. Second, it eases screening for function of rescued plant sequences following transformation of wild-type cells. Coupled with Agrobacterium protoplast cocultivation, activation tagging could also be applied to generate cell lines, and ultimately plant lines, selected for growth under any biochemical selection. This approach can also be used to produce cell lines to grow in the absence of cytokinin or in the presence of selective levels of an inhibitor of polyamine biosynthesis. This suggests that the procedure may have general application. An advantage of the approach is that the selection does not require a detailed understanding of the process under investigation. Hence, the mutations obtained could be applied to study poorly understood processes, such as the molecular basis of plant hormone action for which mutants are not available at the present time." @default.
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- W1498454975 date "1995-01-01" @default.
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- W1498454975 title "Chapter 32 Induction of Signal Transduction Pathways through Promoter Activation" @default.
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- W1498454975 doi "https://doi.org/10.1016/s0091-679x(08)61473-9" @default.
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