Matches in SemOpenAlex for { <https://semopenalex.org/work/W1498494026> ?p ?o ?g. }
Showing items 1 to 97 of
97
with 100 items per page.
- W1498494026 endingPage "2691" @default.
- W1498494026 startingPage "2684" @default.
- W1498494026 abstract "Summary Plant microbiota (the microorganisms that live in any associations with plant tissues) represents a rather unexplored area of metagenomic research compared with soils and oceans. Constructing a metagenomic library for plant microbiota is technically challenging. Using all the biomass without pre‐enrichment could lead to vast proportions of the host plant DNA in the metagenomic library, doubtless obliterating the microbial contribution. Therefore, the first and essential step is to enrich for the constituent microorganisms from plant tissues. Here, a strong enrichment for plant microbiota was achieved by coupling SDS (sodium dodecyl sulfate) with NaCl, creating a predominantly microbial metagenomic library that contains 88% bacterial inserts. 16S rDNA sequence analysis revealed that the metagenomic DNA of enrichments originates from very diverse microorganisms. At least 74 distinct ribotypes (at a 97% threshold) from seven different bacterial phyla were identified and mainly distributed among Actinobacteria and Proteobacteria . Additionally, a simplified version of Amplified Ribosomal DNA Restriction Analysis (ARDRA) was developed for a quick and efficient assessment of the enriching procedures. This work opens further insight into the great biotechnical potential of plant microbiota, holding more potential for drug discovery through a metagenomic strategy, and paving the way for recovery and biochemical characterization of functional gene repertoire from plant microbiota." @default.
- W1498494026 created "2016-06-24" @default.
- W1498494026 creator A5005728746 @default.
- W1498494026 creator A5040166946 @default.
- W1498494026 creator A5057891226 @default.
- W1498494026 creator A5073205162 @default.
- W1498494026 date "2008-09-10" @default.
- W1498494026 modified "2023-10-16" @default.
- W1498494026 title "Enriching plant microbiota for a metagenomic library construction" @default.
- W1498494026 cites W1674900236 @default.
- W1498494026 cites W1957158838 @default.
- W1498494026 cites W1968413588 @default.
- W1498494026 cites W1994762405 @default.
- W1498494026 cites W2047598109 @default.
- W1498494026 cites W2052639391 @default.
- W1498494026 cites W2055043387 @default.
- W1498494026 cites W2068687524 @default.
- W1498494026 cites W2072812562 @default.
- W1498494026 cites W2078851068 @default.
- W1498494026 cites W2103498669 @default.
- W1498494026 cites W2107974372 @default.
- W1498494026 cites W2124010228 @default.
- W1498494026 cites W2126809954 @default.
- W1498494026 cites W2131186249 @default.
- W1498494026 cites W2133731751 @default.
- W1498494026 cites W2147783737 @default.
- W1498494026 cites W2156492633 @default.
- W1498494026 cites W2162315106 @default.
- W1498494026 cites W2169929748 @default.
- W1498494026 doi "https://doi.org/10.1111/j.1462-2920.2008.01689.x" @default.
- W1498494026 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18631363" @default.
- W1498494026 hasPublicationYear "2008" @default.
- W1498494026 type Work @default.
- W1498494026 sameAs 1498494026 @default.
- W1498494026 citedByCount "57" @default.
- W1498494026 countsByYear W14984940262012 @default.
- W1498494026 countsByYear W14984940262013 @default.
- W1498494026 countsByYear W14984940262014 @default.
- W1498494026 countsByYear W14984940262015 @default.
- W1498494026 countsByYear W14984940262016 @default.
- W1498494026 countsByYear W14984940262017 @default.
- W1498494026 countsByYear W14984940262018 @default.
- W1498494026 countsByYear W14984940262019 @default.
- W1498494026 countsByYear W14984940262020 @default.
- W1498494026 countsByYear W14984940262022 @default.
- W1498494026 countsByYear W14984940262023 @default.
- W1498494026 crossrefType "journal-article" @default.
- W1498494026 hasAuthorship W1498494026A5005728746 @default.
- W1498494026 hasAuthorship W1498494026A5040166946 @default.
- W1498494026 hasAuthorship W1498494026A5057891226 @default.
- W1498494026 hasAuthorship W1498494026A5073205162 @default.
- W1498494026 hasConcept C104317684 @default.
- W1498494026 hasConcept C1436000 @default.
- W1498494026 hasConcept C15151743 @default.
- W1498494026 hasConcept C175605896 @default.
- W1498494026 hasConcept C2778169975 @default.
- W1498494026 hasConcept C2779676784 @default.
- W1498494026 hasConcept C42062724 @default.
- W1498494026 hasConcept C523546767 @default.
- W1498494026 hasConcept C54355233 @default.
- W1498494026 hasConcept C70721500 @default.
- W1498494026 hasConcept C86803240 @default.
- W1498494026 hasConcept C89423630 @default.
- W1498494026 hasConceptScore W1498494026C104317684 @default.
- W1498494026 hasConceptScore W1498494026C1436000 @default.
- W1498494026 hasConceptScore W1498494026C15151743 @default.
- W1498494026 hasConceptScore W1498494026C175605896 @default.
- W1498494026 hasConceptScore W1498494026C2778169975 @default.
- W1498494026 hasConceptScore W1498494026C2779676784 @default.
- W1498494026 hasConceptScore W1498494026C42062724 @default.
- W1498494026 hasConceptScore W1498494026C523546767 @default.
- W1498494026 hasConceptScore W1498494026C54355233 @default.
- W1498494026 hasConceptScore W1498494026C70721500 @default.
- W1498494026 hasConceptScore W1498494026C86803240 @default.
- W1498494026 hasConceptScore W1498494026C89423630 @default.
- W1498494026 hasIssue "10" @default.
- W1498494026 hasLocation W14984940261 @default.
- W1498494026 hasLocation W14984940262 @default.
- W1498494026 hasOpenAccess W1498494026 @default.
- W1498494026 hasPrimaryLocation W14984940261 @default.
- W1498494026 hasRelatedWork W193764388 @default.
- W1498494026 hasRelatedWork W1976709164 @default.
- W1498494026 hasRelatedWork W1988111326 @default.
- W1498494026 hasRelatedWork W2004730880 @default.
- W1498494026 hasRelatedWork W2012364528 @default.
- W1498494026 hasRelatedWork W2310132469 @default.
- W1498494026 hasRelatedWork W2329998112 @default.
- W1498494026 hasRelatedWork W2356075577 @default.
- W1498494026 hasRelatedWork W2369806802 @default.
- W1498494026 hasRelatedWork W3142000368 @default.
- W1498494026 hasVolume "10" @default.
- W1498494026 isParatext "false" @default.
- W1498494026 isRetracted "false" @default.
- W1498494026 magId "1498494026" @default.
- W1498494026 workType "article" @default.