FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1498560711> ?p ?o ?g. }

• W1498560711 endingPage "114" @default.
• W1498560711 startingPage "103" @default.
• W1498560711 abstract "To facilitate the isolation of externally exposed plasma membrane proteins we have improved a method that utilizes the non-permeant alkylating reagent trinitrobenzene sulfonate (TNBS). Biosynthetically labeled cells were incubated with TNBS using conditions which minimized penetration into the cells and cell damage (10 mM TNBS, pH 7.8 in PBS; 30 min at 4 °C). The viable cells were incubated with rabbit anti-dinitrophenyl (DNP) IgG. After removal of the excess IgG, the cells were lysed in non-ionic detergent and the anti-DNP IgG-TNP-protein complexes were isolated by adsorption with fixed Staphylococcus aureus or protein-A Sepharose. Proteins thus isolated were analysed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). When the cells were first treated with TNBS and then metabolically labeled, a hitherto unsuspected major contamination by adsorbed non-derivatized proteins was observed. Under these conditions the TNBS-derivatized proteins should not be metabolically labeled. The contaminating proteins were not removed by washing the immunoadsorbent with deoxycholate, digitonin, saponin, pH 11 carbonate buffer, or 1.5 M ammonium thiocyanate. However, the non-specifically adsorbed proteins were removed by washing with an SDS/NP-40 mixture (0.1% SDS, 0.05% NP-40, 0.6 M NaCl, 10 mM Tris-HCl, pH 8.6). After having assessed and reduced the extent of contamination by non-derivatized proteins, we compared metabolically labeled TNP-proteins isolated by this procedure with anti-macrophage antiserum immunoprecipitates. [35S]Methionine-labeled proteins isolated by the TNBS method were also compared with total 125I surface labeled proteins, and with 125I-labeled surface proteins isolated after TNBS derivatization and immunoprecipitation. Analysis of autoradiograms of SDS-PAGE gels showed that all of these methods resulted in the identification of remarkably similar sets of proteins. There was consistent agreement between 125I-labeled surface proteins and [35S]methionine-labeled proteins isolated from different cell types, including thioglycollate-elicited macrophages, the J774 macrophage cell line, mouse L cells, and Chinese hamster ovary cells (CHO). Proteins of thioglycollate-elicited macrophages labeled with [35S]methionine, [1-14C]mannose, and [1-14C]-glucosamine were isolated after TNBS derivatization and gave identical gel profiles, suggesting that most if not all of the surface proteins isolated by this method were glycosylated." @default.
• W1498560711 created "2016-06-24" @default.
• W1498560711 creator A5047944765 @default.
• W1498560711 creator A5057584079 @default.
• W1498560711 creator A5085574965 @default.
• W1498560711 creator A5090415157 @default.
• W1498560711 date "1981-05-01" @default.
• W1498560711 modified "2023-09-23" @default.
• W1498560711 title "Studies on externally disposed plasma membrane proteins" @default.
• W1498560711 cites W1570776822 @default.
• W1498560711 cites W1588885776 @default.
• W1498560711 cites W1625660792 @default.
• W1498560711 cites W1936480433 @default.
• W1498560711 cites W1964513734 @default.
• W1498560711 cites W1969380114 @default.
• W1498560711 cites W1970101963 @default.
• W1498560711 cites W1977884403 @default.
• W1498560711 cites W1981881021 @default.
• W1498560711 cites W1987026337 @default.
• W1498560711 cites W1990198778 @default.
• W1498560711 cites W1996234404 @default.
• W1498560711 cites W2002350625 @default.
• W1498560711 cites W2022335022 @default.
• W1498560711 cites W2022611929 @default.
• W1498560711 cites W2034277598 @default.
• W1498560711 cites W2040399575 @default.
• W1498560711 cites W2042624944 @default.
• W1498560711 cites W2054584898 @default.
• W1498560711 cites W2075032348 @default.
• W1498560711 cites W2082564460 @default.
• W1498560711 cites W2089807619 @default.
• W1498560711 cites W2149886980 @default.
• W1498560711 cites W2150712546 @default.
• W1498560711 cites W2154318575 @default.
• W1498560711 cites W2170389082 @default.
• W1498560711 cites W981195140 @default.
• W1498560711 doi "https://doi.org/10.1016/0014-4827(81)90361-x" @default.
• W1498560711 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7016551" @default.
• W1498560711 hasPublicationYear "1981" @default.
• W1498560711 type Work @default.
• W1498560711 sameAs 1498560711 @default.
• W1498560711 citedByCount "12" @default.
• W1498560711 crossrefType "journal-article" @default.
• W1498560711 hasAuthorship W1498560711A5047944765 @default.
• W1498560711 hasAuthorship W1498560711A5057584079 @default.
• W1498560711 hasAuthorship W1498560711A5085574965 @default.
• W1498560711 hasAuthorship W1498560711A5090415157 @default.
• W1498560711 hasConcept C116084860 @default.
• W1498560711 hasConcept C146543888 @default.
• W1498560711 hasConcept C147789679 @default.
• W1498560711 hasConcept C185592680 @default.
• W1498560711 hasConcept C2778913249 @default.
• W1498560711 hasConcept C2778920010 @default.
• W1498560711 hasConcept C2781014771 @default.
• W1498560711 hasConcept C40875361 @default.
• W1498560711 hasConcept C41625074 @default.
• W1498560711 hasConcept C43617362 @default.
• W1498560711 hasConcept C55493867 @default.
• W1498560711 hasConcept C57409179 @default.
• W1498560711 hasConcept C86803240 @default.
• W1498560711 hasConceptScore W1498560711C116084860 @default.
• W1498560711 hasConceptScore W1498560711C146543888 @default.
• W1498560711 hasConceptScore W1498560711C147789679 @default.
• W1498560711 hasConceptScore W1498560711C185592680 @default.
• W1498560711 hasConceptScore W1498560711C2778913249 @default.
• W1498560711 hasConceptScore W1498560711C2778920010 @default.
• W1498560711 hasConceptScore W1498560711C2781014771 @default.
• W1498560711 hasConceptScore W1498560711C40875361 @default.
• W1498560711 hasConceptScore W1498560711C41625074 @default.
• W1498560711 hasConceptScore W1498560711C43617362 @default.
• W1498560711 hasConceptScore W1498560711C55493867 @default.
• W1498560711 hasConceptScore W1498560711C57409179 @default.
• W1498560711 hasConceptScore W1498560711C86803240 @default.
• W1498560711 hasIssue "1" @default.
• W1498560711 hasLocation W14985607111 @default.
• W1498560711 hasLocation W14985607112 @default.
• W1498560711 hasOpenAccess W1498560711 @default.
• W1498560711 hasPrimaryLocation W14985607111 @default.
• W1498560711 hasRelatedWork W1502019371 @default.
• W1498560711 hasRelatedWork W1572746801 @default.
• W1498560711 hasRelatedWork W1967555063 @default.
• W1498560711 hasRelatedWork W1987356413 @default.
• W1498560711 hasRelatedWork W1990549102 @default.
• W1498560711 hasRelatedWork W2011403137 @default.
• W1498560711 hasRelatedWork W2011707458 @default.
• W1498560711 hasRelatedWork W2018451041 @default.
• W1498560711 hasRelatedWork W2811278406 @default.
• W1498560711 hasRelatedWork W4234478048 @default.
• W1498560711 hasVolume "133" @default.
• W1498560711 isParatext "false" @default.
• W1498560711 isRetracted "false" @default.
• W1498560711 magId "1498560711" @default.
• W1498560711 workType "article" @default.