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• W1498789021 abstract "In order to define the differences in the distribution of cytosolic free Ca2+ ([Ca2+]i) in pancreatic beta-cells stimulated with the fuel secretagogue glucose or the Ca(2+)-mobilizing agents carbachol and ATP, we applied digital video imaging to beta-cells loaded with fura-2.83% of the cells responded to glucose with an increase in [Ca2+]i after a latency of 117 +/- 24 s (mean +/- S.E., 85 cells). Of these cells, 16% showed slow wave oscillations (frequency 0.35/min). In order to assess the relationship between membrane potential and the distribution of the [Ca2+]i rise, digital image analysis and perforated patch-clamp methods were applied simultaneously. The system used allowed sufficient temporal resolution to visualize a subplasmalemmal Ca2+ transient due to a single glucose-induced action potential. Glucose could also elicit a slow depolarization which did not cause Ca2+ influx until the appearance of the first of a train of action potentials. [Ca2+]i rose progressively during spike firing. Inhibition of Ca2+ influx by EGTA abolished the glucose-induced rise in [Ca2+]i. In contrast, the peak amplitude of the [Ca2+]i response to carbachol was not significantly different in normal or in Ca(2+)-deprived medium. Occasionally, the increase of the [Ca2+]i rise was polarized to one area of the cell different from the subplasmalemmal rise caused by glucose. The amplitude of the response and the number of responding cells were significantly increased when carbachol was applied after the addition of high glucose (11.2 mM). ATP also raised [Ca2+]i and promoted both Ca2+ mobilization and Ca2+ influx. The intracellular distribution of [Ca2+]i was homogeneous during the onset of the response. A polarity in the [Ca2+]i distribution could be detected either in the descending phase of the peak or in subsequent peaks during [Ca2+]i oscillations caused by ATP. In the absence of extracellular Ca2+, the sequential application of ATP and carbachol revealed that carbachol was still able to raise [Ca2+]i after exhaustion of the ATP response. This may be due to desensitization to the former agonist, since the response occurred in the same area of the cell. These results reveal subtle differences in [Ca2+]i distribution following membrane depolarization with glucose or the application of Ca(2+)-mobilizing agonists." @default.
• W1498789021 created "2016-06-24" @default.
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• W1498789021 date "1992-09-01" @default.
• W1498789021 modified "2023-10-16" @default.
• W1498789021 title "Video imaging of cytosolic Ca2+ in pancreatic beta-cells stimulated by glucose, carbachol, and ATP." @default.
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• W1498789021 cites W1543532453 @default.
• W1498789021 cites W1549576466 @default.
• W1498789021 cites W1555617925 @default.
• W1498789021 cites W1566092281 @default.
• W1498789021 cites W1568228057 @default.
• W1498789021 cites W1595907824 @default.
• W1498789021 cites W1597390857 @default.
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• W1498789021 cites W191377311 @default.
• W1498789021 cites W1964355587 @default.
• W1498789021 cites W1965005201 @default.
• W1498789021 cites W1967086988 @default.
• W1498789021 cites W1969706311 @default.
• W1498789021 cites W1971578810 @default.
• W1498789021 cites W1973533455 @default.
• W1498789021 cites W1977307439 @default.
• W1498789021 cites W1978007520 @default.
• W1498789021 cites W1978598042 @default.
• W1498789021 cites W1978778326 @default.
• W1498789021 cites W1984746596 @default.
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• W1498789021 cites W1992045485 @default.
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• W1498789021 cites W1994302710 @default.
• W1498789021 cites W1994924615 @default.
• W1498789021 cites W2004644115 @default.
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• W1498789021 cites W2031878584 @default.
• W1498789021 cites W2032015223 @default.
• W1498789021 cites W2032487852 @default.
• W1498789021 cites W2036441475 @default.
• W1498789021 cites W2040015414 @default.
• W1498789021 cites W2040336234 @default.
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• W1498789021 cites W2044187333 @default.
• W1498789021 cites W2048505930 @default.
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• W1498789021 doi "https://doi.org/10.1016/s0021-9258(19)37159-5" @default.
• W1498789021 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1517243" @default.
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