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- W1499334540 abstract "Multiple forms of class III DNA-dependent RNA polymerase have been found in a number of higher eukaryotic cell types (Chambon, P. (1975) Annu. Rev. Biochem. 44, 613–638). Similar multiple forms are reported here from a lower eukaryote, the soil amoeba Acanthamoeba castellanii. The levels of activity of all three RNA polymerase classes in whole cell extracts of Acanthamoeba during cellular differentiation were examined. In contrast to our previous observation (Detke, S. and Paule, M.R. (1975) Biochim. Biophys. Acta 383, 67–77) that the amount of the α-amanitin-sensitive polymerases (II + III) increase in the nucleus during encystment we find that the amount of polymerases II and III when solubilized from whole cells remains constant. The level of activity of polymerase I, however, remains constant both in nuclei and in whole cell extracts. Thus, the shutdown of ribosomal RNA synthesis occurring during encystment is not due to a decrease in the number of polymerase I molecules in the cell." @default.
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- W1499334540 date "1978-08-01" @default.
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- W1499334540 title "DNA-dependent RNA polymerases from Acanthamoeba castellanii" @default.
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- W1499334540 doi "https://doi.org/10.1016/0005-2787(78)90014-x" @default.
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